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Find video protocols related to scientific articles indexed in Pubmed.
Roles of miR-497 and its potential signaling pathway in diseases and with vascular endothelial growth factor.
J. Recept. Signal Transduct. Res.
PUBLISHED: 11-05-2014
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Abstract MicroRNA (miRNA) is a class of small endogenous non-coding RNAs that are ??22 nucleotides in length and can have structural, enzymatic and post-transcriptional regulators of gene expression targeting mRNA for translational repression and/or degradation. miR-497 is high on the list of noncoding, small, regulatory RNAs that plays important roles in the pathogenesis of some diseases and takes part in some signaling pathways in some diseases, but many questions await answers. Vascular endothelial growth factor (VEGF) is a notable chemokine that plays critical roles in angiogenesis and vasculogenesis. There might be an association between miRNA-497 and VEGF. This review was performed to sum up the roles of miR-497 and its potential signaling pathway in diseases and with VEGF.
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Demethyleneberberine, a natural mitochondria-targeted antioxidant, inhibits mitochondrial dysfunction, oxidative stress and steatosis in alcoholic liver disease mouse model.
J. Pharmacol. Exp. Ther.
PUBLISHED: 10-31-2014
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Excessive alcohol consumption induces oxidative stress and lipid accumulation in liver. Mitochondria have long been recognized as the key target for alcoholic liver disease (ALD). Recently artificial mitochondria-targeted antioxidant MitoQ has been used to treat ALD effectively in mice. Here we introduce a natural mitochondria-targeted antioxidant Demethyleneberberine (DMB) which has been found in Chinese herb Cortex Phellodendri chinensis. The protective effect of DMB on ALD was evaluated with HepG 2 cells and acutely/chronically ethanol-fed mice, mimicking two common patterns of drinking in human. The results showed that DMB, which is composed of a potential antioxidant structure, could penetrate the membrane of mitochondria and accumulate in mitochondria either in vitro or vivo. Consequently, the acute drinking-caused oxidative stress and mitochondrial dysfunction were significantly ameliorated by DMB. Moreover, we also found that DMB suppressed cytochrome P450 2E1 (CYP2E1), hypoxia inducible factor ? (HIF-1?) and inducible nitric oxide synthase (iNOS) which contributed to oxidative stress and restored sirtuin 1 (SIRT1)/AMP-activated protein kinase (AMPK)/peroxisome proliferator-activated receptor-gamma Co-activator-1? (PGC-1?) pathway-associated fatty acid oxidation in chronic ethanol-fed mice, which in turn ameliorated lipid peroxidation and macrosteatosis in liver. Take together these findings, DMB could serve as a novel and potential therapy for ALD in human beings.
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Cord blood C-peptide, insulin, HbA1c, and lipids levels in small- and large-for-gestational-age newborns.
Med. Sci. Monit.
PUBLISHED: 10-31-2014
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Small- and large-for-gestational-age (SGA, LGA) newborns are associated with metabolic syndrome in their later life. Cord blood C-peptide, insulin, glycosylated hemoglobin (HbA1c), and lipids levels may be altered in SGA and LGA newborns; however, the results are conflicting. Therefore, this study aimed to determine the effect of cord blood markers on SGA and LGA newborns.
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Colorectal cancer incidence and mortality in china, 2010.
Asian Pac. J. Cancer Prev.
PUBLISHED: 10-24-2014
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The National Central Cancer Registry of China (NCCR) affiliated to the Bureau of Disease Control, National Health and Family Planning Commission of China is responsible for cancer surveillance in the entire country. Cancer registration data from each local registry located in each province are collected by NCCR annually to be analyzed and published to provide useful information for policy makers and cancer researchers.
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[Preparation and investigation of MRI-traceable Eudragit-E liquid embolic agent].
Beijing Da Xue Xue Bao
PUBLISHED: 10-22-2014
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To develop and investigate the properties of MRI-traceable Eudragit-E liquid embolic agent (MR-E).
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Wheat-specific gene, ribosomal protein l21, used as the endogenous reference gene for qualitative and real-time quantitative polymerase chain reaction detection of transgenes.
J. Agric. Food Chem.
PUBLISHED: 10-17-2014
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Wheat-specific ribosomal protein L21 (RPL21) is an endogenous reference gene suitable for genetically modified (GM) wheat identification. This taxon-specific RPL21 sequence displayed high homogeneity in different wheat varieties. Southern blots revealed 1 or 3 copies, and sequence analyses showed one amplicon in common wheat. Combined analyses with sequences from common wheat (AABBDD) and three diploid ancestral species, Triticum urartu (AA), Aegilops speltoides (BB), and Aegilops tauschii (DD), demonstrated the presence of this amplicon in the AA genome. Using conventional qualitative polymerase chain reaction (PCR), the limit of detection was 2 copies of wheat haploid genome per reaction. In the quantitative real-time PCR assay, limits of detection and quantification were about 2 and 8 haploid genome copies, respectively, the latter of which is 2.5-4-fold lower than other reported wheat endogenous reference genes. Construct-specific PCR assays were developed using RPL21 as an endogenous reference gene, and as little as 0.5% of GM wheat contents containing Arabidopsis NPR1 were properly quantified.
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Risk factors for complications of pancreatic extracorporeal shock wave lithotripsy.
Endoscopy
PUBLISHED: 09-24-2014
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Background and study aims: Extracorporeal shock wave lithotripsy is recommended as treatment for stones in chronic pancreatitis. The aim of this study was to investigate the risk factors for complications of pancreatic extracorporeal shock wave lithotripsy (P-ESWL). Patients and methods: Patients with painful chronic pancreatitis and pancreatic stones (>?5?mm diameter) who were treated with P-ESWL between March 2011 and June 2013 were prospectively included. Adverse events after P-ESWL were classified as complications and transient adverse events, depending on severity. The major complications of P-ESWL included post-ESWL pancreatitis, bleeding, infection, steinstrasse, and perforation. Multivariate analyses based on univariate analysis were performed to detect risk factors of overall and moderate-to-severe complications. Results: A total of 634 patients underwent 1470 P-ESWL procedures. The overall complication rate was 6.7?% of all procedures. Complications occurred in 62 patients (9.8?%) after the first ESWL procedure. The risk factors for complications were pancreas divisum (odds ratio [OR] 1.28) and the interval between diagnosis of chronic pancreatitis and P-ESWL (OR 1.28). Protective factors were male sex (OR 0.50), diabetes (OR 0.45), and steatorrhea (OR 0.43). Male sex, the only identified predictor for moderate-to-severe complications, was a protective factor (OR 0.19). For the second P-ESWL procedure, complications occurred in 22/409 patients (5.4?%). Complication and asymptomatic hyperamylasemia after the first ESWL session were significantly associated with higher risk for complications after the second ESWL session (P?
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[Release of mercury from soil and plant in water-level-fluctuating zone of the Three Gorges Reservoir area and its accumulation in zebrafish].
Huan Jing Ke Xue
PUBLISHED: 09-24-2014
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To investigate the production, distribution and bioavailability of methylmercury (MMHg) in soil and plants of the water-level-fluctuating zone (WLFZ) of the Three Gorges Reservoir area, simulation experiments were conducted in laboratory. Results indicated that the level of total mercury (THg) in soil decreased with the lengthening of submerging time while that in water increased obviously. The level of MMHg in inundated soil and water increased, especially in the water treated by Echinochloa crusgalli and soils. And the MMHg level in that treatment was 2.52 times higher than that treated only by soils for 21 days. This indicated that soil and plants of WLFZ were important sources of mercury in the water of the reservoir. Echinochloa crusgalli as the tested plant was decomposed after being submerged, leading to lower pH and DO and higher DOC, which had little effect on MMHg in soil but significant effect on MMHg in water. The level of THg in the head, viscera and muscle of zebrafish increased obviously, which had a significant correlation with that in water (P < 0.01). MMHg levels accumulated in the head, viscera and muscle of zebrafish differed to some degree, particularly in the head and muscle. After treated in the soils for 21 days, MMHg levels in the head, viscera and muscle of zebrafish were 1.75-6.25, 3.53-8.38 and 2.22-3.36 times higher than those in the control groups, respectively. While for the treatment of Echinochloa crusgalli and soil, MMHg levels in zebrafish's head, viscera and muscle were 3.57, 2.37 and 1.52 times higher than those treated only by soil, respectively. Therefore, submerged soil was the main source of MMHg in fish. And submerged plants changed the water condition and affected the release of mercury to water so as to cause elevated levels of MMHg in fish.
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Borealin/Dasra B is overexpressed in colorectal cancers and contributes to proliferation of cancer cells.
Med. Oncol.
PUBLISHED: 08-27-2014
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Borealin/Dasra B, also called cell division cycle associated 8, is a member of the chromosomal passenger complex and plays a crucial role during mitosis and cell division. The expression of this gene has been investigated in several kinds of cancers, but its role in colorectal cancers (CRC) remains to be elucidated. In this study, our in silico analysis using the Oncomine database showed that Borealin was overexpressed in CRC tissues, which was further validated by immunohistochemistry in a cohort of 81 pairs of primary CRC and adjacent non-cancerous tissues. In CRC, Borealin expression was positively correlated with age (P = 0.0079), lymph node metastasis (P = 0.0086), and TNM stage (P = 0.0050). Significant difference in the overall survival time was seen between patients with high and low expression (P < 0.0001). Furthermore, high Borealin expression remained independently prognostic in multivariate analysis (HR 4.556, 95 % CI 1.964-10.567, P = 0.0004). Functional studies showed that suppression of Borealin expression with siRNA could significantly inhibit the growth and induce apoptosis in HCT-116 cells (P < 0.01). These results suggest that Borealin might act as an independent prognostic factor and a potential therapeutic target for patients with CRC.
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[The clinical analysis for the whole-spine magnetic resonance imaging of axial spondyloarthritis].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 08-23-2014
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To investigate spinemagnetic resonance imaging(MRI) findings in patients with axial spondyloarthritis (SpA) and to analyze the correlation between imaging and clinical manifestation.
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Synthesis, antibacterial activity, and biological evaluation of formyl hydroxyamino derivatives as novel potent peptide deformylase inhibitors against drug-resistant bacteria.
Eur J Med Chem
PUBLISHED: 08-12-2014
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Peptide deformylase (PDF) has been identified as a promising target for novel antibacterial agents. In this study, a series of novel formyl hydroxyamino derivatives were designed and synthesized as PDF inhibitors and their antibacterial activities were evaluated. Among the potent PDF inhibitors (1o, 1q, 1o', 1q', and 1x), in vivo studies showed that compound 1q possesses mild toxicity, a good pharmacokinetic profile and protective effects. The good in vivo efficacy and low toxicity suggest that this class of compounds has potential for development and use in future antibacterial drugs.
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Optimization of reference genes for normalization of the quantitative polymerase chain reaction in tissue samples of gastric cancer.
Asian Pac. J. Cancer Prev.
PUBLISHED: 08-02-2014
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For an exact comparison of mRNA transcription in different samples or tissues with real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR), it is crucial to select a suitable internal reference gene. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin (ACTB) have been frequently considered as house-keeping genes to normalize for changes in specific gene expression. However, it has been reported that these genes are unsuitable references in some cases, because their transcription is significantly variable under particular experimental conditions and among tissues. The present study was aimed to investigate which reference genes are most suitable for the study of gastric cancer tissues using qRT-PCR. 50 pairs of gastric cancer and corresponding peritumoral tissues were obtained from patients with gastric cancer. Absolute qRT-PCR was employed to detect the expression of GAPDH, ACTB, RPII and 18sRNA in the gastric cancer samples. Comparing gastric cancer with corresponding peritumoral tissues, GAPDH, ACTB and RPII were obviously up-regulated 6.49, 5.0 and 3.68 fold, respectively. Yet 18sRNA had no obvious expression change in gastric cancer tissues and the corresponding peritumoral tissues. The expression of GAPDH, ?-actin, RPII and 18sRNA showed no obvious changes in normal gastric epithelial cells compared with gastric cancer cell lines. The carcinoembryonic antigen (CEA), a widely used clinical tumor marker, was used as a validation gene. Only when 18sRNA was used as the normalizing gene was CEA obviously elevated in gastric cancer tissues compared with peritumoral tissues. Our data show that 18sRNA is stably expressed in gastric cancer samples and corresponding peritumoral tissues. These observations confirm that there is no universal reference gene and underline the importance of specific optimization of potential reference genes for any experimental condition.
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Comparative study on immediate versus delayed meniscus allograft transplantation: 4- to 6-year follow-up.
Am J Sports Med
PUBLISHED: 07-28-2014
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Meniscus allograft transplantation (MAT) is generally used for patients who sustain symptoms after meniscectomy, which could be called delayed MAT. Meniscus allograft transplantation for patients immediately after meniscectomy has not been reported.
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[Research advances on immunoregulation and anti-inflammation function of mesenchymal stem cells and their application in treatment of renal diseases].
Zhejiang Da Xue Xue Bao Yi Xue Ban
PUBLISHED: 07-08-2014
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Mesenchymal stem cell (MSC) can differentiate into multiple lines in various tissues. MSC has the advantage of congenital hypoimmunogenicity and can interact with both innate and adaptive immunocytes, exerting immunoregulatory function via direct cell-cell contact and secreting soluble factors. It also can migrate to tissue injury sites to dampen inflammatory reactions. MSC can be potentially applied to treat immunological and inflammatory diseases, such as acute renal injury, immune kidney diseases, diabetic nephropathy and end-stage renal diseases. This review summarizes the biological characteristics of MSC and the prospects of its application in treatment of renal diseases.
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Forest runoff increase mercury output from subtropical forest catchments: an example from an alpine reservoir in a national nature reserve (southwestern China).
Environ Sci Pollut Res Int
PUBLISHED: 06-24-2014
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For a typical alpine forest reservoir that does not have other obvious mercury pollution sources, mercury levels in its downstream water can reflect the characteristics of mercury inputs and risks of a remote forest. To confirm this proposal, eight sampling campaigns were carried out in 2012 and 2013 to investigate the distribution patterns of Hg species in the water column and sediment profiles at four sampling stations in an alpine forest reservoir, southwestern China. The concentrations of total Hg (THg) and methylmercury (MeHg) in water samples of DaHong Reservoir varied from 2.8 to 8.2 ng L(-1) and 0.06 to 2.9 ng L(-1), respectively. The concentrations of these Hg species in the wet seasons (summer and fall) were significantly higher than those in the dry season (winter and spring). Forest field runoff and diffusion of Hg from sediments could be the reasons for elevated concentrations of these Hg species in the wet season. Elevated MeHg concentrations in water samples from the bottom water and water-sediment interface demonstrated an active net Hg methylation in the downstream of DaHong (DH) Reservoir. Dissolved MeHg levels in the pore water of surface sediments and the bottom of downstream were apparently higher than those in the upstream, which indicated that MeHg sources of sediment pore water varied in space. MeHg diffusive fluxes from sediment to overlying water were higher in the wet season than those in the dry season, demonstrating that high temperatures favor Hg methylation processes in sediment.
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Integrating analysis reveals microRNA-mediated pathway crosstalk among Crohn's disease, ulcerative colitis and colorectal cancer.
Mol Biosyst
PUBLISHED: 06-21-2014
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Inflammatory bowel disease (IBD), which can increase the risk of colorectal cancer (CRC), includes two primary subtypes, ulcerative colitis (UC) and Crohn's disease (CD). Although several individual genes involved in inflammation or cancer characterization have been identified, it is still difficult to elucidate functional relationship details between the molecules underlying pathogenesis at the system level. The global effect of miRNAs on genes or their involved functions is also poorly understood. We first integrated genome-wide gene expression profiles and biological pathway information to explore the underlying associations among UC, CD and CRC at the function and gene level. After identifying the pathways regulated by miRNAs, a global map of miRNA-mediated pathway crosstalk shared by the three diseases was further constructed to vertically explain the links of three level alterations. The three types of diseases have close associations with each other at the levels of function, gene and miRNA regulation. Several key biological pathways are involved in the three diseases, related to the immune system and inflammation, metabolism, or cell proliferation and apoptosis etc. Moreover, miRNAs exhibit dominant effects on multiple pathways. It is worth noting that UC shows relatively close associations with CD and CRC at the three levels. Finally, the miRNAs could mediate the crosstalk within or between pathways. For example, hsa-miR-125b, hsa-miR-335 and hsa-miR-155 mediated the crosstalk between three metabolic pathways. The crosstalk within the Toll-like receptor signaling pathway could be mediated by hsa-miR-124, hsa-miR-146a and hsa-mir-221/222. Our results make sense for the prevention and treatment of intestinal-related chronic inflammation or cancer.
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Wet deposition flux and runoff output flux of mercury in a typical small agricultural watershed in Three Gorges Reservoir areas.
Environ Sci Pollut Res Int
PUBLISHED: 06-20-2014
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In order to explore the contributions of wet deposition and runoff formed by rainfall events to the water body mercury burden in Three Gorges Reservoir (TGR), we conducted a 1-year successive study on the deposition fluxes and runoff output characteristics of total mercury (THg) and methylmercury (TMeHg) in a typical small agricultural watershed in TGR areas. The results showed that the annual volume-weighted concentration (VWC) of THg and TMeHg was 18 and 0.23 ng L(-1), respectively. Particulate form was the main form of both THg and TMeHg, accounting for 61 % of THg and 59 % of TMeHg. The annual deposition fluxes of THg and TMeHg in rainfall were 13?±?2.4 ?g m(-2) year(-1) and 174?±?52 ng m(-2) year(-1), respectively. The VWCs of THg and TMeHg in runoff were 10?±?6.5 and 0.15?±?0.15 ng L(-1). The annual output fluxes of THg and TMeHg to TGR from study area were 1.2?±?0.73 ?g m(-2) year(-1) and 17?±?16 ng m(-2) year(-1), respectively.
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Design, synthesis and biological activity of flavonoid derivatives as selective agonists for neuromedin U 2 receptor.
Bioorg. Med. Chem.
PUBLISHED: 06-16-2014
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Central neuromedin U 2 receptor (NMU2R) plays important roles in the regulation of food intake and body weight. Identification of NMU2R agonists may lead to the development of pharmaceutical agents to treat obesity. Based on the structure of rutin, a typical flavonoid and one of the NMU2R agonists we previously identified from an in-house made natural product library, 30 flavonoid derivatives have been synthesized and screened on a cell-based reporter gene assay. A number of compounds were found to be selective and highly potent to NMU2R. For example, the EC50 value of compound NRA 4 is very close to that of NMU, the endogenous peptide ligand of NMU2R. Structure-activity relationship analysis revealed that a 3-hydroxyl group in ring C and a 2'-fluoride group in ring B were essential for this class of compounds to be active against NMU2R.
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Candidate lesion-based criteria for defining a positive sacroiliac joint MRI in two cohorts of patients with axial spondyloarthritis.
Ann. Rheum. Dis.
PUBLISHED: 06-14-2014
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To determine candidate lesion-based criteria for a positive sacroiliac joint (SIJ) MRI based on bone marrow oedema (BMO) and/or erosion in non-radiographic axial spondyloarthritis (nr-axSpA); to compare the performance of lesion-based criteria with global evaluation by expert readers.
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[Ultraviolet-visible (UV-Vis) spectral characteristics of dissolved organic matter (DOM) in soils and sediments of typical water-level fluctuation zones of Three Gorges Reservoir areas].
Huan Jing Ke Xue
PUBLISHED: 06-03-2014
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Dissolved organic matter (DOM) is a very important component in terrestrial ecosystem, which also plays a key role in geo-environmental chemistry. A number of DOM samples were extracted from soils and sediments samples obtained from typical water-level fluctuation zones of Three Gorges reservoir areas. Ultraviolet-visible (UV-Vis) absorption spectra were recorded for unveiling the geochemical characteristics of DOM based on specific absorption parameters and fitting models. Through the results of specific absorption parameters, it was suggested that the aromaticity, hydrophobicity and humification degree were lower in soils than in the surface sediments, which were also independent of the sampling location height and land-use types. Among the four absorption models selected, model 2 was the optimal. Meanwhile, different models and fitting wavelengths also significantly affected the absorption spectral slope (S value): S increased with the decreasing wavelength. Additionally, the correlation among different S values obtained from different fitting wavelength ranges was significantly different suggesting that the S values in various wavelength ranges only indicated the tip of the iceberg of DOM characteristics instead of the entirety. Furthermore, the ratio of specific spectral slope (S(R)) indicated lower degradation (photo-bleaching or microbial degradation) degree in the surface sediments as compared to those in soils and older sediments. The surface sediments had higher aromaticity, hydrophobicity, molecular weight and photochemical/ biodegradation activity potentials, suggesting fresher DOM, lower effect of photobleaching and microbial degradation activities.
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[Comparation of the transperitoneal and extraperitoneal laparoscopic radical prostatectomy].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 05-10-2014
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To compare the transperitoneal laparoscopic radical prostatectomy procedures with extraperitoneal laparoscopic radical prostatectomy procedures to assess for differences in outcomes and complications.
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3.0 T conventional hip MR and hip MR arthrography for the acetabular labral tears confirmed by arthroscopy.
Eur J Radiol
PUBLISHED: 05-04-2014
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To evaluate the value of hip MR for diagnosing acetabular labrum tears, and to further compare the diagnostic performances of conventional MR with MR arthrography in acetabular labrum tears.
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Exploration of type II binding mode: A privileged approach for kinase inhibitor focused drug discovery?
ACS Chem. Biol.
PUBLISHED: 04-29-2014
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The ATP site of kinases displays remarkable conformational flexibility when accommodating chemically diverse small molecule inhibitors. The so-called activation segment, whose conformation controls catalytic activity and access to the substrate binding pocket, can undergo a large conformational change with the active state assuming a 'DFG-in' and an inactive state assuming a 'DFG-out' conformation. Compounds that preferentially bind to the DFG-out conformation are typically called 'type II' inhibitors in contrast to 'type I' inhibitors that bind to the DFG-in conformation. This review surveys the large number of type II inhibitors that have been developed and provides an analysis of their crystallographically determined binding modes. Using a small library of type II inhibitors, we demonstrate that more than 200 kinases can be targeted, suggesting that type II inhibitors may not be intrinsically more selective than type I inhibitors.
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Expression, Purification, and Characterization of Scar Tissue Neovasculature Endothelial Cell-Targeted rhIL10 in Escherichia Coli.
Appl. Biochem. Biotechnol.
PUBLISHED: 04-25-2014
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Interleukin 10 (IL10) plays a pivotal role in the anti-inflammatory response and immunosuppressive reactions. It has also been identified as a new promising therapy for scar formation. Treatment of scars with IL10 has significant effects, but there are some shortcomings, including poor tissue-binding specificity and low effectiveness. RGD peptide has been demonstrated to bind specifically to ?v?3 integrin on neovasculature endothelial cells, and the excess production of neovasculature is crucial to scar formation. To increase efficacy against scar formation and to decrease the side effects on normal tissues, a novel hybrid protein combining human IL10 with RGD was designed. The DNA sequence encoding the recombinant fusion protein IL10-RGD (rhIL10-RGD) was subcloned into a pET22b (+) vector for protein expression in E. coli strain BL21 (DE3). SDS-PAGE analysis displayed an induced expression product band at a molecular weight of 19.3 kDa, which constituted 30 % of the total bacterial protein. We developed a procedure to purify rhIL10-RGD from inclusion bodies and then renatured the protein using dialysis against urea with a step-down concentration procedure. Hypertrophic scar fibroblasts (HSFs) were treated with rhIL10-RGD, and the fibrosis-related protein levels were assessed by Western blotting. The results indicated that rhIL10-RGD can downregulate the expression levels of Col1 and ?-SMA in HSFs and suppress tube formation of HUVECs. These results indicate that rhIL10-RGD has anti-fibrosis effects and can potentially be used to treat the neovasculature in scar formation and improve the abnormal deposition of the extracellular matrix (ECM). Thus, rhIL10-RGD may be a more effective candidate for scar-improvement and anti-fibrosis therapy.
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Effect of maternal lipid profile, C-peptide, insulin, and HBA1c levels during late pregnancy on large-for-gestational age newborns.
World J Pediatr
PUBLISHED: 04-04-2014
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Large-for-gestational age (LGA) newborns can increase the risk of metabolic syndrome. Previous studies have shown that the levels of maternal blood lipids, connecting peptide (C-peptide), insulin and glycosylated hemoglobin (HbA1c) were significantly different between LGA and appropriate-for-gestational age (AGA) newborns. This study aimed to determine the effect of the levels of maternal lipids, C-peptide, insulin, and HbA1c during late pregnancy on LGA newborns.
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Effect of pulmonary vein isolation on atrial fibrillation recurrence after ablation of paroxysmal supraventricular tachycardia in patients with high dispersion of atrial refractoriness.
J Interv Card Electrophysiol
PUBLISHED: 04-01-2014
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This study aimed to assess pulmonary vein isolation (PVI) efficacy on atrial fibrillation (AF) recurrence and to determine a predictive dispersion of atrial refractoriness (dERP) value for performing PVI in paroxysmal supraventricular tachycardia (PSVT) patients.
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Discovery of a potent, covalent BTK inhibitor for B-cell lymphoma.
ACS Chem. Biol.
PUBLISHED: 03-17-2014
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BTK is a member of the TEC family of non-receptor tyrosine kinases whose deregulation has been implicated in a variety of B-cell-related diseases. We have used structure-based drug design in conjunction with kinome profiling and cellular assays to develop a potent, selective, and irreversible BTK kinase inhibitor, QL47, which covalently modifies Cys481. QL47 inhibits BTK kinase activity with an IC50 of 7 nM, inhibits autophosphorylation of BTK on Tyr223 in cells with an EC50 of 475 nM, and inhibits phosphorylation of a downstream effector PLC?2 (Tyr759) with an EC50 of 318 nM. In Ramos cells QL47 induces a G1 cell cycle arrest that is associated with pronounced degradation of BTK protein. QL47 inhibits the proliferation of B-cell lymphoma cancer cell lines at submicromolar concentrations.
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Scientific publications in respiratory journals from Chinese authors in various parts of North Asia: a 10-year survey of literature.
BMJ Open
PUBLISHED: 03-04-2014
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Respiratory disease remains one of the leading causes of morbidity and mortality in China. However, little is known about the research status of respirology in three major regions of China-Mainland (ML), Hong Kong (HK) and Taiwan (TW). A 10-year survey of literature was conducted to compare the three regions' outputs in the research of respirology.
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Functional and pathological improvements of the hearts in diabetes model by the combined therapy of bFGF-loaded nanoparticles with ultrasound-targeted microbubble destruction.
J Control Release
PUBLISHED: 02-27-2014
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Diabetic cardiomyopathy (DCM) is the leading cause of morbidity and mortality among the diabetic patients and currently there is no effective means to reverse its pathological progress. Basic fibroblast growth factor (bFGF) has shown promise as a molecular therapy for DCM, but its delivery is inefficient and non-specific. In the present study, a therapy combining nanoparticle (NP) carrier and ultrasound-targeted microbubble destruction (UTMD) was reported the first time for bFGF delivery to the heart of diabetic rats. bFGF-loaded NP (bFGF-NP) were prepared with Poloxamer 188-grafted heparin copolymer using water-in-water technique, and the morphology, encapsulation efficiency, and bioactivity of bFGF-NP were studied. The cellular uptake and cytotoxicity of bFGF-NP were evaluated with primary cultures of the left ventricular (LV) cardiomyocytes in vitro. Therapeutic effects of bFGF-NP/UTMD on the heart of DCM rats were studied by measuring LV systolic and diastolic functions, hemodynamic characteristics and indicators of cardiac remodeling including myocardial collagen volume fraction and capillary density. Results demonstrated that bFGF-NP showed good round morphology, efficient bFGF encapsulation and stable bioactivity of bFGF in vitro. bFGF-NP/UTMD combined treatment significantly enhanced the efficiency of bFGF cellular uptake (P<0.05) without obvious cytotoxicity. Significant improvements (P<0.05) in both cardiac functions and tissue morphology in the DCM rats were observed in bFGF-NP/UTMD group. These were not achievable using free bFGF, bFGF-NP or UTMD treatment alone. Our results show that combining a non-viral vector with UTMD technique is an effective strategy to deliver bFGF to the heart, and the resulting growth factor therapy has demonstrated potential to reverse the progress of DCM by restoring the cardiac functions and even the structure of damaged cardiac tissues.
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Preparation and microscopy examination of alginate-poly-l-lysine-alginate microcapsules.
Drug Dev Ind Pharm
PUBLISHED: 02-25-2014
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Abstract Ca-alginate-poly-l-lysine-alginate (APA-Ca) and Ba-alginate-poly-l-lysine-alginate (APA-Ba) microcapsules were prepared and their thickness and surface were examined by light microscopy and scanning electron microscopy. Specifically, light microscopy with frozen section was used to visualize and quantify the thickness of APA membrane, and monitor temporal changes in the thickness of microcapsules during a month long culture in vitro. The section graph of APA microcapsule represents the accurate measurement of layer thickness of APA-Ca with diameter 900?±?100 and 500?±?100??m at 6.01?±?1.02 and 9.54?±?2.42??m (p??0.05), except that the recovery of APA-Ba was higher than the APA-Ca microcapsules. The paper showed an easy method to prepare APA-Ca and APA-Ba, and examine their thickness and surface, which could be utilized to study other types of microcapsules.
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Platelets induce a proinflammatory phenotype in monocytes via the CD147 pathway in rheumatoid arthritis.
Arthritis Res. Ther.
PUBLISHED: 02-19-2014
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IntroductionActivated platelets exert a proinflammatory action that can be largely ascribed to their ability to interact with monocytes. However, the mechanisms that promote dynamic changes in monocyte subsets in rheumatoid arthritis (RA) have not been clearly identified. The aim of this study was to determine whether platelet activation and the consequent formation of monocyte-platelet aggregates (MPA) might induce a proinflammatory phenotype in circulating monocytes in RA.MethodsThe surface phenotype of platelets and the frequencies of monocyte subpopulations in the peripheral blood of RA patients were determined using flow cytometry. Platelets were sorted and co-cultured with monocytes. In addition, monocyte activation was assessed by measuring the nuclear factor ¿B (NF-¿B) pathway. The disease activity was evaluated using the 28-joint disease activity score.ResultsPlatelet activation, circulating intermediate monocytes (Mon2) and MPA formation were significantly elevated in RA, especially in those with active disease status. Furthermore, Mon2 monocytes showed higher CD147 expression and responded to direct cell contact with activated platelets with higher cytokine production and matrix metallopeptidase 9 (MMP-9) secretion, which increased the expression of CD147. After the addition of specific antibodies for CD147, those effects were abolished. Furthermore, the NF-¿B¿driven inflammatory pathway may be involved in this process.ConclusionThese findings indicate an important role of platelet activation and the consequent formation of MPA in the generation of the proinflammatory cytokine milieu and for the promotion and maintenance of the pathogenically relevant Mon2 monocyte compartment in RA, which is likely to play an important role in the pathogenesis of autoimmunity.
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Development and evaluation of liquid embolic agents based on liquid crystalline material of glyceryl monooleate.
Int J Pharm
PUBLISHED: 02-08-2014
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New type of liquid embolic agents based on a liquid crystalline material of glyceryl monooleate (GMO) was developed and evaluated in this study. Ternary phase diagram of GMO, water and ethanol was constructed and three isotropic liquids (ILs, GMO:ethanol:water=49:21:30, 60:20:20 and 72:18:10 (w/w/w)) were selected as potential liquid embolic agents, which could spontaneously form viscous gel cast when contacting with water or physiological fluid. The ILs exhibited excellent microcatheter deliverability due to low viscosity, and were proved to successfully block the saline flow when performed in a device to simulate embolization in vitro. The ILs also showed good cytocompatibility on L929 mouse fibroblast cell line. The embolization of ILs to rabbit kidneys was performed successfully under monitoring of digital subtraction angiography (DSA), and embolic degree was affected by the initial formulation composition and used volume. At 5th week after embolization, DSA and computed tomography (CT) confirmed the renal arteries embolized with IL did not recanalize in follow-up period, and an obvious atrophy of the embolized kidney was observed. Therefore, the GMO-based liquid embolic agents showed feasible and effective to embolize, and potential use in clinical interventional embolization therapy.
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[Efficacy and safety of a phenylalanine-free amino acid-based enteral formula (AA-PKU2) in 1-8 year-old children with phenylketonuria: a prospective, open, self-controlled and multi-center study in China].
Zhongguo Dang Dai Er Ke Za Zhi
PUBLISHED: 01-28-2014
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To evaluate the efficacy and safety of a phenylalanine-free amino acid-based enteral formula (AA-PKU2) in the treatment of children with phenylketonuria (PKU) aged 1-8 years.
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Does spinal MRI add incremental diagnostic value to MRI of the sacroiliac joints alone in patients with non-radiographic axial spondyloarthritis?
Ann. Rheum. Dis.
PUBLISHED: 01-24-2014
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To assess the incremental diagnostic value of spine MRI evaluated separately from and combined with sacroiliac joint (SIJ) MRI in non-radiographic axial spondyloarthritis (nr-axSpA) compared with SIJ MRI alone.
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Repair of large full-thickness cartilage defect by activating endogenous peripheral blood stem cells and autologous periosteum flap transplantation combined with patellofemoral realignment.
Knee
PUBLISHED: 01-11-2014
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Minimal-invasive procedure and one-step surgery offer autologous mesenchymal stem cells derived from peripheral blood (PB-MSCs) a promising prospective in the field of cartilage regeneration. We report a case of a 19-year-old male athlete of kickboxing with ICRS grade IV chondral lesions at the 60° region of lateral femoral trochlea, which was repaired by activating endogenous PB-MSCs plus autologous periosteum flap transplantation combined with correcting the patellofemoral malalignment. After a 7.5 year follow-up, the result showed that the patient returned to competitive kickboxing. Second-look under arthroscopy showed a smooth surface at 8 months postoperation. The IKDC 2000 subjective score, Lysholm score and Tegner score were 95, 98 and 9 respectively at the final follow up. CT and MRI evaluations showed a significant improvement compared with those of pre-operation.
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Anti-fibrotic actions of interleukin-10 against hypertrophic scarring by activation of PI3K/AKT and STAT3 signaling pathways in scar-forming fibroblasts.
PLoS ONE
PUBLISHED: 01-01-2014
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The hypertrophic scar (HS) is a serious fibrotic skin condition and a major clinical problem. Interleukin-10 (IL-10) has been identified as a prospective scar-improving compound based on preclinical trials. Our previous work showed that IL-10 has anti-fibrotic effects in transforming growth factor (TGF)-?1-stimulated fibroblasts, as well as potential therapeutic benefits for the prevention and reduction of scar formation. However, relatively little is known about the mechanisms underlying IL-10-mediated anti-fibrotic and scar-improvement actions.
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Current approaches to enhance CNS delivery of drugs across the brain barriers.
Int J Nanomedicine
PUBLISHED: 01-01-2014
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Although many agents have therapeutic potentials for central nervous system (CNS) diseases, few of these agents have been clinically used because of the brain barriers. As the protective barrier of the CNS, the blood-brain barrier and the blood-cerebrospinal fluid barrier maintain the brain microenvironment, neuronal activity, and proper functioning of the CNS. Different strategies for efficient CNS delivery have been studied. This article reviews the current approaches to open or facilitate penetration across these barriers for enhanced drug delivery to the CNS. These approaches are summarized into three broad categories: noninvasive, invasive, and miscellaneous techniques. The progresses made using these approaches are reviewed, and the associated mechanisms and problems are discussed.
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Comparative study of sex differences in distal femur morphology in osteoarthritic knees in a Chinese population.
PLoS ONE
PUBLISHED: 01-01-2014
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The purpose of this study was to investigate sex differences in resected distal femoral morphology in Chinese osteoarthritic knees.
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[The clinical characteristics of 26 cases of amyopathic dermatomyositis].
Zhonghua Nei Ke Za Zhi
PUBLISHED: 11-26-2013
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To analyze the clinical characteristics of amyopathic dermatomyositis (ADM).
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Tetracyanodibenzotetrathiafulvalene diimides: design, synthesis, and property study.
J. Org. Chem.
PUBLISHED: 11-12-2013
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Tetracyanodibenzotetrathiafulvalene diimide (TCDBTTF-DI), an isomer of core-expanded naphthalene diimides bearing two 2-(1,3-dithiol-2-ylidene)malononitrile moieties (NDI-DTYM2), has been designed and synthesized to explore the effect of its isomeric structure on the optical and electrochemical properties of the materials. UV-vis spectra show that TCDBTTF-DI exhibits variation in its absorption peaks while maintaining a similar optical band gap to NDI-DTYM2. Electrochemical studies indicate that TCDBTTF-DI can not only accept but also lose electrons, in contrast to the solely electron-accepting behavior of NDI-DTYM2.
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Comprehensive analysis of the functional microRNA-mRNA regulatory network identifies miRNA signatures associated with glioma malignant progression.
Nucleic Acids Res.
PUBLISHED: 11-04-2013
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Glioma is the most common and fatal primary brain tumour with poor prognosis; however, the functional roles of miRNAs in glioma malignant progression are insufficiently understood. Here, we used an integrated approach to identify miRNA functional targets during glioma malignant progression by combining the paired expression profiles of miRNAs and mRNAs across 160 Chinese glioma patients, and further constructed the functional miRNA-mRNA regulatory network. As a result, most tumour-suppressive miRNAs in glioma progression were newly discovered, whose functions were widely involved in gliomagenesis. Moreover, three miRNA signatures, with different combinations of hub miRNAs (regulations?30) were constructed, which could independently predict the survival of patients with all gliomas, high-grade glioma and glioblastoma. Our network-based method increased the ability to identify the prognostic biomarkers, when compared with the traditional method and random conditions. Hsa-miR-524-5p and hsa-miR-628-5p, shared by these three signatures, acted as protective factors and their expression decreased gradually during glioma progression. Functional analysis of these miRNA signatures highlighted their critical roles in cell cycle and cell proliferation in glioblastoma malignant progression, especially hsa-miR-524-5p and hsa-miR-628-5p exhibited dominant regulatory activities. Therefore, network-based biomarkers are expected to be more effective and provide deep insights into the molecular mechanism of glioma malignant progression.
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[Diagnosis and treatment of primary colorectal non-Hodgkins lymphoma: analysis of 52 cases].
Zhonghua Zhong Liu Za Zhi
PUBLISHED: 08-30-2013
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To summarize and analyze the diagnosis, clinical features and therapy of primary colorectal non-Hodgkins lymphoma (NHL).
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[Systemic therapy for defects of skin and soft tissue on the knees after severe trauma or burn].
Zhonghua Shao Shang Za Zhi
PUBLISHED: 08-30-2013
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To explore the methods of systemical treatment of defects of skin and soft tissue on the knees after severe trauma or burn.
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Discovery of a potent and selective DDR1 receptor tyrosine kinase inhibitor.
ACS Chem. Biol.
PUBLISHED: 08-13-2013
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The DDR1 receptor tyrosine kinase is activated by matrix collagens and has been implicated in numerous cellular functions such as proliferation, differentiation, adhesion, migration, and invasion. Here we report the discovery of a potent and selective DDR1 inhibitor, DDR1-IN-1, and present the 2.2 Å DDR1 co-crystal structure. DDR1-IN-1 binds to DDR1 in the DFG-out conformation and inhibits DDR1 autophosphorylation in cells at submicromolar concentrations with good selectivity as assessed against a panel of 451 kinases measured using the KinomeScan technology. We identified a mutation in the hinge region of DDR1, G707A, that confers >20-fold resistance to the ability of DDR1-IN-1 to inhibit DDR1 autophosphorylation and can be used to establish what pharmacology is DDR1-dependent. A combinatorial screen of DDR1-IN-1 with a library of annotated kinase inhibitors revealed that inhibitors of PI3K and mTOR such as GSK2126458 potentiate the antiproliferative activity of DDR1-IN-1 in colorectal cancer cell lines. DDR1-IN-1 provides a useful pharmacological probe for DDR1-dependent signal transduction.
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Evaluation of efficacy and safety of sunitinib regimen in 22 patients with metastatic renal cell carcinoma: at least 12-month follow-up.
Chin. Med. J.
PUBLISHED: 08-09-2013
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Sunitinib has been proved an effective new option for treatment of metastatic renal cell carcinoma (mRCC). Analysis of clinical data of 22 patients, who were exposed to sunitinib for at least 1 year, was conducted to evaluate the long-term efficacy and safety of sunitinib for the treatment of mRCC.
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The FAt Spondyloarthritis Spine Score (FASSS): development and validation of a new scoring method for the evaluation of fat lesions in the spine of patients with axial spondyloarthritis.
Arthritis Res. Ther.
PUBLISHED: 08-07-2013
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Studies have shown that fat lesions follow resolution of inflammation in the spine of patients with axial spondyloarthritis (SpA). Fat lesions at vertebral corners have also been shown to predict development of new syndesmophytes. Therefore, scoring of fat lesions in the spine may constitute both an important measure of treatment efficacy as well as a surrogate marker for new bone formation. The aim of this study was to develop and validate a new scoring method for fat lesions in the spine, the Fat SpA Spine Score (FASSS), which in contrast to the existing scoring method addresses the localization and phenotypic diversity of fat lesions in patients with axial SpA.
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[Correlation of lumbar disc degeneration and spinal-pelvic sagittal balance].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-02-2013
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To elucidate the relationship between spino-pelvic sagittal balance parameters and lumbar intervertebral disc degeneration of each segment through retrospective analysis in lumbar degeneration patients.
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[Expression of Osterix mRNA and protein induced by recombinant human bone morphogenetic protein-2 in human periodontal ligament cells].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 08-02-2013
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To detect the changes of Osterix (Osx) mRNA and protein expression in human periodontal ligament cells (HPDLCs) induced by recombinant human bone morphogenetic protein-2 (rhBMP-2), and examine the role of BMP-2 and Osx during the osteogenic differentiation of HPDLCs.
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Gelatin nanostructured lipid carriers-mediated intranasal delivery of basic fibroblast growth factor enhances functional recovery in hemiparkinsonian rats.
Nanomedicine
PUBLISHED: 07-27-2013
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Lipid nanoparticles with solid matrix have been given increasing attention due to their biodegradable status and ability to entrap a variety of biologically active compounds. In this study, new phospholipid-based gelatin nanoparticles encapsulating basic fibroblast growth factor (bFGF) were developed to target the brain via nasal administration. Treatment effects were assessed by quantifying rotational behavior, monoamine neurotransmitter levels and tyrosine hydroxylase expression in 6-hydroxydopamine induced hemiparkinsonian rats. The gelatin nanostructured lipid carriers (GNLs) were prepared by a water-in-water emulsion method and then freeze-dried. The GNLs possessed better profile than gelatin nanoparticles (GNs), with particle size 143±1.14nm and Zeta potential -38.2±1.2mV. The intranasal GNLs efficiently enriched exogenous bFGF in olfactory bulb and striatum without adverse impact on the integrity of nasal mucosa and showed obvious therapeutic effects on hemiparkinsonian rats. Thus, GNLs are attractive carriers for nose-to-brain drug delivery, especially for unstable macromolecular drugs such as bFGF.
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Walking the interactome to identify human miRNA-disease associations through the functional link between miRNA targets and disease genes.
BMC Syst Biol
PUBLISHED: 07-18-2013
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MicroRNAs (miRNAs) are important post-transcriptional regulators that have been demonstrated to play an important role in human diseases. Elucidating the associations between miRNAs and diseases at the systematic level will deepen our understanding of the molecular mechanisms of diseases. However, miRNA-disease associations identified by previous computational methods are far from completeness and more effort is needed.
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[The diagnosis, treatment and prognosis evaluation of gastric small cell carcinoma: analysis of 41 cases].
Zhonghua Wai Ke Za Zhi
PUBLISHED: 07-18-2013
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To summarize and analyze the clinical feature, therapeutic methods and prognosis of gastric small cell carcinoma (SCC).
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A 5-fluorouracil-loaded polydioxanone weft-knitted stent for the treatment of colorectal cancer.
Biomaterials
PUBLISHED: 07-06-2013
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In-stents restenosis caused by tumour ingrowth is a major problem for patients undergoing stent displacement because the conventional stents often lack a sustained anti-tumour capability. The aim of this paper was to develop a weft-knitted polydioxanone stent which can slow release 5-fluorouracil (5-FU). In order to determine the most suitable drug concentration, the 5-FU safe concentration in vivo and appropriate loading percentage in the membranes were investigated, and then 5-FU-loaded poly-l-lactide membranes at concentration of 3.2%, 6.4% and 12.8% were coated onto the stent using electro-spinning method, respectively. The morphology, chemical structure and in vitro drug release property of the coating membranes were subsequently examined. Their anti-tumour activity and mechanism were assessed in vitro and in vivo using a human colorectal cancer cell line HCT-116 and tumour-bearing BALB/c nude mice. The half maximal inhibitory concentration (IC50) and the median lethal dose (LD50) demonstrated that the 6.4% and 12.8% membranes had better anti-tumour effects than pure 5-FU due to the sustainable drug releasing property of the coated membranes on the stent. The membranes possessing appropriate drug loading doses, such as 6.4% or 12.8% also provided better anti-in-stents restenosis effects than other groups tested. Therefore, it is concluded that the drug-loaded stents have great potential for the use in the treatment of intestinal cancers in the future.
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Glycolipid metabolic status of overweight/obese adolescents aged 9- to 15-year-old and the BMI-SDS/BMI cut-off value of predicting dyslipidemiain boys, Shanghai, China: a cross-sectional study.
Lipids Health Dis
PUBLISHED: 06-27-2013
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The prevalence of adolescents obesity and overweight has dramatically elevated in China. Obese children were likely to insulin resistance and dyslipidemia, which are risk factors of cardiovascular diseases. However there was no cut-off point of anthropometric values to predict the risk factors in Chinese adolescents. The present study was to investigate glycolipid metabolism status of adolescents in Shanghai and to explore the correlations between body mass index standard deviation score (BMI-SDS) and metabolic indices, determine the best cut-off value of BMI-SDS to predict dyslipidemia.
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Clinical and prognostic implications of serum uric acid levels on IgA nephropathy: a cohort study of 348 cases with a mean 5-year follow-up.
Clin. Nephrol.
PUBLISHED: 06-26-2013
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To assess the prognostic implications of serum uric acid levels on patients with IgA nephropathy in a longitudinal 8-year follow-up study and to identify an association between serum uric acid levels and the clinical and pathological phenotypes of IgA nephropathy.
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Clinicopathologic characteristics and prognostic factors of 63 gastric cancer patients with metachronous ovarian metastasis.
Cancer Biol Med
PUBLISHED: 05-18-2013
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This study aims to explore the clinicopathologic characteristics and prognostic factors of gastric cancer patients with metachronous ovarian metastasis.
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[Effect of Osterix overexpression on osteogenic differentiation of human periodontal ligament cells].
Hua Xi Kou Qiang Yi Xue Za Zhi
PUBLISHED: 05-14-2013
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To investigate the effects of Osterix (Osx) overexpression on the osteogenic differentiation of human periodontal ligament cells in response to mechanical force.
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Discovery of a Selective Irreversible BMX Inhibitor for Prostate Cancer.
ACS Chem. Biol.
PUBLISHED: 04-26-2013
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BMX is a member of the TEC family of nonreceptor tyrosine kinases. We have used structure-based drug design in conjunction with kinome profiling to develop a potent, selective, and irreversible BMX kinase inhibitor, BMX-IN-1, which covalently modifies Cys496. BMX-IN-1 inhibits the proliferation of Tel-BMX-transformed Ba/F3 cells at two digit nanomolar concentrations but requires single digit micromolar concentrations to inhibit the proliferation of prostate cancer cell lines. Using a combinatorial kinase inhibitor screening strategy, we discovered that the allosteric Akt inhibitor, MK2206, is able to potentiate BMX inhibitors antiproliferation efficacy against prostate cancer cells.
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Soluble Tie2 fusion protein decreases peritoneal angiogenesis in uremic rats.
Mol Med Rep
PUBLISHED: 04-23-2013
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Angiogenesis is considered to be one of the most common mechanisms leading to ultrafiltration failure (UFF) in long-term peritoneal dialysis (PD) patients. The angiopoietin (Ang)/Tie system was found to play a role in the initiation of pathological neoangiogenesis and is also involved in peritoneal angiogenesis caused by peritoneal fluid. This aim of this study was to investigate the effects of the soluble Tie2 fusion protein (sTie2/Fc) on peritoneal angiogenesis in PD-treated uremic rats. The rats were divided into 6 groups: normal, sham surgery, uremic rats without PD, uremic PD-treated rats, uremic rats treated with PD and sTie2/Fc (0.25 µg/100 g) and uremic rats treated with PD and sTie2/Fc (0.5 µg/100 g). PD rats were treated once a day for 28 days prior to testing. Real-time polymerase chain reaction (RT-PCR) or tissue immunohistochemical staining was used to detect Ang-2 mRNA or protein expression in the peritoneal tissues of each group. The microvessel density (MVD) of the peritoneum was detected and quantified by immunohistochemical staining using the anti-CD34 antibody. Compared with the control group, Ang-2 mRNA and protein expression was significantly upregulated in the uremic and PD groups (P<0.05). MVD in the experimental group increased compared with the control group. sTie2/Fc treatment decreased the levels of Ang-2 mRNA and protein expression (P<0.05) in a dose-dependent manner and decreased PD-induced MVD in the peritoneum. In conclusion, angiogenesis of the peritoneum induced by PD was inhibited using sTie2/Fc in a uremic rat model.
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Spatial distribution and pollution assessment of mercury in sediments of Lake Taihu, China.
J Environ Sci (China)
PUBLISHED: 04-20-2013
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Spatial distribution patterns of total mercury (THg) in 36 surficial sediment samples representing five regions of Lake Taihu were assessed using the ArcGis geostatistical analyst module. The pollution levels of THg were also evaluated from the same five lake regions. Concentrations of THg were in a ranged of 23-168 ng/g (mean 55 ng/g) in surfical sediments, which was significantly higher than those established baseline levels of the lake. Results of THg indicated that the northern region exhibited notably higher values, the bay regions showed elevated values relative to open areas, and the lakeside regions were higher than those observed in the central area. Lake Taihu suffered moderate to high Hg pollution, and expressed clear Hg enrichment status according to monomial pollution index I(geo) and human activity factors. The concentrations of THg in the surficial sediments of Lake Taihu showed moderate-strong variation (coefficient of variation 52%). Geostatistical analysis indicated a weak spatial self-correlation, suggesting the contamination of Hg in Lake Taihu is primarily the result of anthropogenic activities.
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Toxicity study of isolated polypeptide from wool hydrolysate.
Food Chem. Toxicol.
PUBLISHED: 03-19-2013
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The cytotoxicity of wool polypeptide has been evaluated by both cell and animal models. Wool was dissolved in sodium hydroxide solution, the pH value of the solution was adjusted to 5.55 and the precipitate was harvested as wool polypeptide. The spray-dried polypeptide was collected as powders and characterized by SEM, FTIR and TG-DSC. The cell culturing results showed that wool polypeptide had no obvious negative effect on cell viability in vitro. Both acute oral toxicity and subacute 30-day oral toxicology studies showed that wool polypeptide had no influence on body weight, feed consumption, blood chemistry, and hematology at any dose levels. There were no treatment related findings on gross or detailed necroscopy, organ weights, organ/body weight ratios and histology. Our study indicated the absence of toxicity in wool polypeptide and supported its safe use as a food ingredient or drug carrier.
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[Methylation analysis of CpG island DNA of FMR1 gene in the fragile X syndrome].
Zhonghua Yi Xue Yi Chuan Xue Za Zhi
PUBLISHED: 03-02-2013
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To establish a method of methylation-sensitive restriction enzymes based quantitative PCR (MSRE-qPCR) for analysis of CpG island DNA of FMR1 gene, and to assess its value for molecular diagnosis of fragile X syndrome.
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Developing irreversible inhibitors of the protein kinase cysteinome.
Chem. Biol.
PUBLISHED: 02-27-2013
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Protein kinases are a large family of approximately 530 highly conserved enzymes that transfer a ?-phosphate group from ATP to a variety of amino acid residues, such as tyrosine, serine, and threonine, that serves as a ubiquitous mechanism for cellular signal transduction. The clinical success of a number of kinase-directed drugs and the frequent observation of disease causing mutations in protein kinases suggest that a large number of kinases may represent therapeutically relevant targets. To date, the majority of clinical and preclinical kinase inhibitors are ATP competitive, noncovalent inhibitors that achieve selectivity through recognition of unique features of particular protein kinases. Recently, there has been renewed interest in the development of irreversible inhibitors that form covalent bonds with cysteine or other nucleophilic residues in the ATP-binding pocket. Irreversible kinase inhibitors have a number of potential advantages including prolonged pharmacodynamics, suitability for rational design, high potency, and ability to validate pharmacological specificity through mutation of the reactive cysteine residue. Here, we review recent efforts to develop cysteine-targeted irreversible protein kinase inhibitors and discuss their modes of recognizing the ATP-binding pocket and their biological activity profiles. In addition, we provided an informatics assessment of the potential "kinase cysteinome" and discuss strategies for the efficient development of new covalent inhibitors.
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Expression of interleukin-17 associated with disease progression and liver fibrosis with hepatitis B virus infection: IL-17 in HBV infection.
Diagn Pathol
PUBLISHED: 02-22-2013
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As a proinflammatory cytokine, interleukin-17 (IL-17) contributes to the inflammation of many autoimmune diseases. We examined IL-17 levels in serum and tissues from patients with chronic hepatitis B virus infection (HBV), and especially evaluated the role of IL-17 in the pathogenesis and progression of liver fibrosis.
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Inhibition of EphA4 expression promotes Schwann cell migration and peripheral nerve regeneration.
Neurosci. Lett.
PUBLISHED: 02-20-2013
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Erythropoietin-producing hepatocellular carcinoma receptors (Ephs) and their ligands Ephrins can affect axon growth. To evaluate the efficacy of EphA4 knockdown on Schwann cell migration and peripheral nerve regeneration, we detected EphA4 levels in Schwann cells. To knock down the expression of EphA4 in Schwann cell, two independent small interfering RNAs (siRNAs) were designed, and Schwann cell migration was examined. Four days after surgery, sciatic nerve sections of wild-type (WT) and EphA4(-/-) rats were examined by immunofluorescence, and axonal outgrowth was analyzed. The EphA4 protein could be detected in Schwann cells from intact nerves. EphA4 mediates the inhibitory effect on Schwann cell migration, and EphA4 knock-down can strongly increase Schwann cell migration and peripheral nerve regeneration. Knocking-down the expression of EphA4 promotes peripheral axon growth in vivo. It may provide a potential strategy for the recovery of peripheral nerve injury.
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Research of novel biocompatible radiopaque microcapsules for arterial embolization.
Int J Pharm
PUBLISHED: 02-17-2013
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Embolic agents, such as microparticles, microspheres or beads used in current embolotherapy are mostly radiolucent, which means the agents are invisible under X-ray imaging during and after the process of embolization, and the fate of these particles cannot be precisely assessed. In this research, a radiopaque embolic agent was developed by encapsulating lipiodol in polyvinyl alcohol. The lipiodol-containing polyvinyl alcohol microcapsules (LPMs) were characterized and evaluated for their morphology, size distribution, lipiodol content, lipiodol release, elasticity, and deliverability through catheter. The radiopacity of LPMs in vials and in living mice was both detected by an X-ray imaging system. The biocompatibility of LPMs was investigated with L929 cells and in mice after subcutaneous injection. Embolization of LPMs to a rabbit kidney was performed under digital subtraction angiography (DSA) and the radiopacity of LPMs was verified by computed tomography (CT).
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Efficacy of tendon stem cells in fibroblast-derived matrix for tendon tissue engineering.
Cytotherapy
PUBLISHED: 02-12-2013
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After injury, tendons often heal with poor tissue quality and inferior mechanical properties. Tissue engineering using tendon stem cells (TSCs) is a promising approach in the repair of injured tendon. Tenogenic differentiation of TSCs needs an appropriate environment. More recently, the acellular extracellular matrix (ECM) generated from fibroblasts has been used to construct various engineering tissues. In this study, we successfully developed an engineered tendon tissue formed by seeding TSCs in de-cellularized fibroblast-derived matrix (dFM).
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Lysophosphatidic acid aberrancies and hepatocellular carcinoma: studies in the MDR2 gene knockout mouse.
Cancer Invest.
PUBLISHED: 02-01-2013
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Studies show that lysophosphatidic acid (LPA) reprogramming is associated with the development of hepatocellular carcinoma (HCC). This manuscript evaluates the MDR2(-/-) model of HCC as a tool to examine the role of LPA reprogramming in the initiation/progression of HCC and identify novel treatment targets. Hepatic tumors developed in MDR2(-/-) mice between 9-12 m and serum LPA levels were greater in MDR2(-/-) when compared to controls. Blocking LPA biosynthesis/signaling significantly reduced tumor burden. LPA biosynthesis/signaling plays an important role in murine MDR2(-/-) model and is potentially linked to regulation of TNF? or other cytokines that are relevant to high-risk patients.
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Selection of high efficient transdermal lipid vesicle for curcumin skin delivery.
Int J Pharm
PUBLISHED: 01-31-2013
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Curcumin shows effective anti-inflammatory activities but is seldom used in clinic because of its poor solubility in water and vulnerablity to sunshine ultraviolet effect. Novel lipid vesicles have been developed as carriers for skin delivery. In this paper, lipid vesicles-propylene glycol liposomes (PGL), Ethosomes and traditional liposomes, were prepared as curcumin carriers respectively. Their morphology, particle size and encapsulation efficiency and drug release behavior in vitro were evaluated. Transdermal efficiency and deposition quantity in abdominal skin were also measured with Franz diffusion device. Carrageenan-induced paw edema was established to evaluate the anti-inflammatory effect. From the result, the particle size order of lipid vesicles was: PGL (182.4 ± 89.2 nm)Ethosomes>traditional liposomes. PGL had the best encapsulation efficiency of 92.74 ± 3.44%. From anti-inflammatory experiment, PGL showed the highest and longest inhibition on the development of paw edema, followed by Ethosomes and Traditional liposomes. With the elevated entrapment efficiency, good transdermic ability and sustained-release behavior, PGL may represent an efficient transdermal lipid vesicle for skin delivery.
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The efficacy and safety of Jitongning Capsule () in patients with ankylosing spondylitis.
Chin J Integr Med
PUBLISHED: 01-31-2013
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To confirm the efficacy and safety of Jitongning Capsule in the treatment of ankylosing spondylitis (AS).
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JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.