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Find video protocols related to scientific articles indexed in Pubmed.
Rapid diversification of five Oryza AA genomes associated with rice adaptation.
Proc. Natl. Acad. Sci. U.S.A.
PUBLISHED: 11-03-2014
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Comparative genomic analyses among closely related species can greatly enhance our understanding of plant gene and genome evolution. We report de novo-assembled AA-genome sequences for Oryza nivara, Oryza glaberrima, Oryza barthii, Oryza glumaepatula, and Oryza meridionalis. Our analyses reveal massive levels of genomic structural variation, including segmental duplication and rapid gene family turnover, with particularly high instability in defense-related genes. We show, on a genomic scale, how lineage-specific expansion or contraction of gene families has led to their morphological and reproductive diversification, thus enlightening the evolutionary process of speciation and adaptation. Despite strong purifying selective pressures on most Oryza genes, we documented a large number of positively selected genes, especially those genes involved in flower development, reproduction, and resistance-related processes. These diversifying genes are expected to have played key roles in adaptations to their ecological niches in Asia, South America, Africa and Australia. Extensive variation in noncoding RNA gene numbers, function enrichment, and rates of sequence divergence might also help account for the different genetic adaptations of these rice species. Collectively, these resources provide new opportunities for evolutionary genomics, numerous insights into recent speciation, a valuable database of functional variation for crop improvement, and tools for efficient conservation of wild rice germplasm.
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Next-Generation Digital PCR Measurement of Hepatitis B Virus Copy Number in Formalin-Fixed Paraffin-Embedded Hepatocellular Carcinoma Tissue.
Clin. Chem.
PUBLISHED: 11-02-2014
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Hepatocellular carcinoma (HCC) is strongly associated with hepatitis B virus (HBV) infection. False-negative results are common in routine serological tests and quantitative real-time PCR because of HBV surface antigen (HBsAg) variation and low HBV copy number. Droplet digital PCR (ddPCR), a next-generation digital PCR, is a novel, sensitive, and specific platform that can be used to improve HBV detection.
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[Development of a portable mid-infrared rapid analyzer for oil concentration in water based on MEMS linear sensor array].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 11-01-2014
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Aiming at the existing problems such as weak environmental adaptability, low analytic efficiency and poor measuring repeatability in the traditional spectral oil analyzers, the present paper designed a portable mid-infrared rapid analyzer for oil concentration in water. To reduce the volume of the instrument, the non-symmetrical folding M-type Czerny-Turner optical structure was adopted in the core optical path. With a periodically rotating chopper, controlled by digital PID algorithm, applied for infrared light modulation, the modulating accuracy reached ±0.5%. Different from traditional grating-scanning spectrophotometers, this instrument used a fixed grating for light dispersion and avoided rotating error in the course of the measuring procedures. A new-type MEMS infrared linear sensor array was applied for modulated spectral signals detection, which improved the measuring efficiency remarkably. Optical simulation and experimental results indicate that the spectral range is 2 800 - 3 200 cm(-1), the spectral resolution is 6 cm(-1) (@3 130 cm(-1)), and the signal to noise ratio is up to 5 200 : 1. The acquisition time is 13 milliseconds per spectrogram, and the standard deviation of absorbance is less than 3 x 10(-3). These performances meet the standards of oil concentration measurements perfectly. Compared with traditional infrared spectral analyzers for oil concentration, the instrument demonstrated in this paper has many advantages such as smaller size, more efficiency, higher precision, and stronger vibration & moisture isolation. In addition, the proposed instrument is especially suitable for the environmental monitoring departments to implement real-time measurements in the field for oil concentration in water, hence it has broad prospects of application in the field of water quality monitoring.
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[Profile analysis and comparison of the Han and Uygur adult in Xinjiang area].
Shanghai Kou Qiang Yi Xue
PUBLISHED: 10-24-2014
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To obtain the profile and difference between the Han and Uygur normal adult in Xinjiang area.
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[Contribution of different processes in wetland soil N2O production in different restoration phases of the Yellow River estuary, China].
Huan Jing Ke Xue
PUBLISHED: 10-24-2014
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By using the method of time-space mutual substitution, the contribution of different processes in wetland soil N2O production was studied in the un-restoration wetland (R0), restoration wetland since 2007 (R2007) and restoration wetland since 2002 (R2002) of the Yellow River estuary to evaluate the effectiveness of the restoration projects. Results showed wetland soil total N2O production had a significant difference in different restoration phases, but the N2O release was the main source. The N2O production in restoration wetland was higher than that in un-restoration wetland. The N2O production wss mainly due to the nitrification and nitrifier denitrification processes, while the denitrification process had great weakening effects on N2O production, which was closely related to the physical and chemical properties of wetland soils in different restoration phases. The non-biological processes made greater contributions to N2O production and these were mainly due to that iron was reductive, while the Yellow River estuary was an area of highly active iron. Although N2O production in wetland soils was the results of biological processes combined with non-biological processes in different restoration phases, non-biological processes had larger influences and should be paid a special attention. There were different influences on wetland soil processes generating N2O between temperature and water content, indicating responses of soil microbial activities to temperature and water content were different. In addition, the N2O production contents ranged from 0.37 +/- 0.08 nmol x (kg x h) (-1) to 9.75 +/- 7.64 nmol x (kg x h) (-1) in marshes of the Yellow River estuary, which was slightly higher than those in the S. alterniflora wetland soils of the Min River estuary, but significantly lower than those in the C. malaccensis wetland soils of the Min River estuary, the grassland soils and the aerobic forest soils. We found that the long-term implements of ecological restoration project in the Yellow River estuary obviously promoted N2O production, so we should consider two factors of landscape restoration and weakening greenhouse gases in the next wetland restoration project.
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Complete mitochondrial genome sequence and mutations of the Glioma model inbred C57BL/6 mice strain.
Mitochondrial DNA
PUBLISHED: 10-17-2014
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Abstract In the present work we undertook the complete mitochondrial genome sequencing of an important glioma model inbred rat strain for the first time. The total length of the mitogenome was 16,308?bp. It harbored 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and 1 non-coding control region (D-loop region). The mutation events were also reported.
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[Influence of electroacupuncture plus jasminoidia intervention on expression of OX-42 and nuclear factor-kappa B in hippocampus and amygdale in cerebral ischemia plus diabetes rats].
Zhen Ci Yan Jiu
PUBLISHED: 09-16-2014
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To observe the changes of expression of OX-42 and nuclear factor-kappa B (NF-kappaB) transporter factor in the hippocampus and amygdale in diabetes and cerebral ischemia (CI) rats with learning and memory impairment after combined treatment with electroacupuncture (EA) plus Jasminaidia, so as to explore their mechanism underlying cerebral protection.
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Distribution and environmental significance of nitrite-dependent anaerobic methane-oxidising bacteria in natural ecosystems.
Appl. Microbiol. Biotechnol.
PUBLISHED: 08-31-2014
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Nitrite-dependent anaerobic methane oxidation (N-DAMO) is a recently discovered process that is performed by "Candidatus Methylomirabilis oxyfera" (M. oxyfera). This process constitutes a unique association between the two major global elements essential to life, carbon and nitrogen, and may act as an important and overlooked sink of the greenhouse gas methane. In recent years, more and more studies have reported the distribution of M. oxyfera-like bacteria and the occurrence of N-DAMO process in different natural ecosystems, including freshwater lakes, rivers, wetlands and marine ecosystems. Previous studies have estimated that a total of 2 %-6 % of current worldwide methane flux in wetlands could be consumed via the N-DAMO process. These findings indicate that N-DAMO is indeed a previously overlooked methane sink in natural ecosystems. Given the worldwide increase in anthropogenic nitrogen pollution, the N-DAMO process as a methane sink in reducing global warming could become more important in the future. The present mini-review summarises the current knowledge of the ecological distribution of M. oxyfera-like bacteria and the potential importance of the N-DAMO process in reducing methane emissions in various natural ecosystems. The potential influence of environmental factors on the N-DAMO process is also discussed.
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The complete mitochondrial genome of domestic sheep, Ovis aries.
Mitochondrial DNA
PUBLISHED: 08-27-2014
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Abstract In this study, we report a complete mitochondrial (mt) genome sequence of the Texel ewe, Ovis aries. The total genome is 16,615?bp in length and its overall base composition was estimated to be 33.68% for A, 27.36% for T, 25.86% for C, and 13.10% for G indicating an AT-rich (61.04%) feature in the O. aries mtgenome. It contains a total of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a control region (D-loop region). Comparisons with other publicly available sheep mitogenomes revealed a bunch of nucleotide diversity. This complete mitgenome sequence would enlarge useful genomic information for further studies on sheep evolution and domestication that will enhance germplasm conservation and breeding programs of O. aries.
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The complete mitochondrial genome of eastern lowland gorilla, Gorilla beringei graueri, and comparative mitochondrial genomics of Gorilla species.
Mitochondrial DNA
PUBLISHED: 08-27-2014
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Abstract In this study, we determined the complete mitochondrial (mt) genome of eastern lowland gorilla, Gorilla beringei graueri for the first time. The total genome was 16,416?bp in length. It contained a total of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 control region (D-loop region). The base composition was A (30.88%), G (13.10%), C (30.89%) and T (25.13%), indicating that the percentage of A+T (56.01%) was higher than G+C (43.99%). Comparisons with the other publicly available Gorilla mitogenome showed the conservation of gene order and base compositions but a bunch of nucleotide diversity. This complete mitochondrial genome sequence will provide valuable genetic information for further studies on conservation genetics of eastern lowland gorilla.
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The complete mitochondrial genome of Celebes wild boar, Sus celebensis (Cetartiodactyla: Suina: Suidae), and comparative mitochondrial genomics of the Sus species.
Mitochondrial DNA
PUBLISHED: 08-27-2014
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Abstract In this study, the complete mitochondrial (mt) genome sequence of Sus celebensis was firstly determined. The total genome was 16,481?bp in length and its overall base composition was estimated to be 34.9% for A, 25.8% for T, 26.2% for C, 13.1% for G, respectively, indicating an A-T (60.7%)-rich feature in Celebes wild boar mitogenome. It harbored 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a non-coding control region (D-loop region). Comparisons with other publicly available pig mitogenomes revealed abundant nucleotide diversity. This complete mitgenome sequence would accelerate further studies on pig evolution and domestication that will enhance germplasm preservation and breeding programs of the pig gene pool.
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Synthesis of magnetically separable Ag3PO4/TiO2/Fe3O4 heterostructure with enhanced photocatalytic performance under visible light for photoinactivation of bacteria.
ACS Appl Mater Interfaces
PUBLISHED: 08-27-2014
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Silver orthophosphate (Ag3PO4) is a low-band-gap photocatalyst that has received considerable research interest in recent years. In this work, the magnetic Ag3PO4/TiO2/Fe3O4 heterostructured nanocomposite was synthesized. The nanocomposite was found to exhibit markedly enhanced photocatalytic activity, cycling stability, and long-term durability in the photodegradation of acid orange 7 (AO7) under visible light. Moreover, the antibacterial film prepared from Ag3PO4/TiO2/Fe3O4 nanocomposite presented excellent bactericidal activity and recyclability toward Escherichia coli (E. coli) cells under visible-light irradiation. In addition to the intrinsic cytotoxicity of silver ions, the elevated bactericidal efficiency of Ag3PO4/TiO2/Fe3O4 can be largely attributed to its highly enhanced photocatalytic activity. The photogenerated hydroxyl radicals and superoxide ions on the formed Ag/Ag3PO4/TiO2 interfaces cause considerable morphological changes in the microorganism's cells and lead to the death of the bacteria.
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Transcriptome analysis of the oil-rich tea plant, Camellia oleifera, reveals candidate genes related to lipid metabolism.
PLoS ONE
PUBLISHED: 08-19-2014
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Rapidly driven by the need for developing sustainable sources of nutritionally important fatty acids and the rising concerns about environmental impacts after using fossil oil, oil-plants have received increasing awareness nowadays. As an important oil-rich plant in China, Camellia oleifera has played a vital role in providing nutritional applications, biofuel productions and chemical feedstocks. However, the lack of C. oleifera genome sequences and little genetic information have largely hampered the urgent needs for efficient utilization of the abundant germplasms towards modern breeding efforts of this woody oil-plant.
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Synergic effect between 5?fluorouracil and celecoxib on hypoxic gastric cancer cells.
Mol Med Rep
PUBLISHED: 08-14-2014
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5?fluorouracil (5?FU) is commonly used in the treatment of gastric cancer; however, resistance to this drug occurs under hypoxic conditions. Celecoxib may be used to reverse this resistance. The aim of the present study was to elucidate the inhibitory effects and mechanisms of 5?FU and celecoxib on the gastric cancer cell line SGC7901 under hypoxic conditions. SGC7901 cells were divided into four groups: Hypoxic control group, 5?FU group, celecoxib group and 5?FU/celecoxib combination group. Following treatment, the inhibition rates of cells were determined using an MTT assay. Protein and messenger RNA (mRNA) expression of hypoxia?inducible factor 2? (HIF?2?), adenosine triphosphate?binding cassette sub?family G member 2 (ABCG2) and octamer binding protein 4 (Oct?4) were determined using immunohistochemistry, reverse transcription quantitative polymerase chain reaction (RT?qPCR) and western blot analysis. The results demonstrated that the 5?FU/celecoxib combination group had a significantly higher inhibition rate than the individually treated 5?FU and celecoxib groups (P<0.05); inhibition rates were 66.09, 52.61 and 46.1%, respectively. mRNA and protein expression levels of HIF?2?, ABCG2 and Oct?4 were significantly lower in the celecoxib and 5?FU/celecoxib combination groups (P<0.01) compared with those of the hypoxia control and 5?FU groups. The 5?FU group demonstrated the highest levels of the respective mRNA and proteins. In conclusion, the results of the present study indicated that celecoxib had anti?tumor effects, as it was shown to inhibit tumor cell growth via the inhibition of HIF?2?, ABCG2 and Oct?4. The 5?FU/celecoxib combination had a synergic effect on tumor growth inhibition. This therefore suggested that inhibition of HIF?2?, ABCG2 and Oct?4 may be a potential method of reducing chemotherapy resistance and enhancing the effectiveness of chemotherapy treatment.
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Development of Amperometric Glucose Biosensor Based on Prussian Blue Functionlized TiO2 Nanotube Arrays.
Sci Rep
PUBLISHED: 08-05-2014
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Amperometric biosensors consisting of oxidase and peroxidase have attracted great attention because of their wide application. The current work demonstrates a novel approach to construct an enzymatic biosensor based on TiO2 nanotube arrays (TiNTs) as a supporting electrode on which Prussian Blue (PB)-an "artificial enzyme peroxidase" and enzyme glucose oxidase (GOx) have been immobilized. For this, PB nanocrystals are deposited onto the nanotube wall photocatalytically using the intrinsic photocatalytical property of TiO2, and the GOx/AuNPs nanobiocomposites are subsequently immobilized into the nanotubes via the electrodeposition of polymer. The resulting electrode exhibits a fast response, wide linear range, and good stability for glucose sensing. The sensitivity of the sensor is as high as 248?mA M(-1) cm(-2), and the detection limit is about 3.2??M. These findings demonstrate a promising strategy to integrate enzymes and TiNTs, which could provide an analytical access to a large group of enzymes for bioelectrochemical applications including biosensors and biofuel cells.
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Plasma Interleukin-10: A Likely Predictive Marker for Hepatitis B Virus-Related Acute-on-Chronic Liver Failure.
Hepat Mon
PUBLISHED: 07-14-2014
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The pathogenesis of HBV-related acute-on-chronic liver failure (HBV-ACLF) is mainly based on a heightened immune-inflammatory reaction; however, the intimate underlying mechanism remains unclear.
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Residual Amount of HBV DNA in Serum is Related to Relapse in Chronic Hepatitis B Patients After Cessation of Nucleos(t)ide Analogs.
J. Clin. Gastroenterol.
PUBLISHED: 07-12-2014
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To investigate the relationship between relapse and the levels of the residual amount of HBV DNA in serum at cessation in chronic hepatitis B patients meeting 2008 Asian Pacific Association for the Study of the Liver (APASL) nucleos(t)ide analogs (NAs) cessation criteria.
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[Study on the best detector-distance of noninvasive biochemical examination by Monte Carlo simulation].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 07-11-2014
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The present paper studies the best detector-distance to improve the near-infrared spectrum signal intensity of the dermis layer and eliminate the interference of the epidermis and subcutaneous layer. First, we analyzed the organizational structure of the skin and calculated the tissue optical parameters of different layers. And we established the Monte Carlo model with the example of glucose absorption peak at 2 270 nm. Then, we used the Monte Carlo method to simulate the light transmission rules in the skin, obtaining the average path length, the average visit depth and the fractions of absorbed energy at each layer with the change in critical angle and detector-distance. The results show that when the photons are incident at an angle less than 45 degrees, you can ignore the effect of the incident angle on photon transmission path, and when the detector-distance is 1 mm, the fraction of absorbed photon energy by the dermis layer is the largest, while it can ensure more energy received by detector. We determined that the best detector-distance is 1mm, which successfully avoids the interference of the epidermis spectral information and obtains large amounts of blood in the dermis layer, which is conducive to the near-infrared non-invasive measurement of biochemical components and the subsequent experiments.
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[Indoor simulation on dew formation on plant leaves].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 07-03-2014
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Dew forming on plant leaves through water condensation plays a significant ecological role in arid and semi-arid areas as an ignorable fraction of water resources. In this study, an artificial intelligent climate chamber and an automatic temperature-control system for leaves were implemented to regulate the ambient temperature, the leaf surface temperature and the leaf inclination for dew formation. The impact of leaf inclination, ambient temperature and dew point-leaf temperature depression on the rate and quantity of dew accumulation on leaf surface were analyzed. The results indicated that the accumulation rate and the maximum volume of dew on leaves decreased with increasing the leaf inclination while increased with the increment of dew point-leaf temperature depression, ambient temperature and relative humidity. Under the horizontal configuration, dew accumulated linearly on leaf surface over time until the maximum volume (0.80 mm) was reached. However, dew would fall down after reaching the maximum volume when the leaf inclination existed (45 degrees or 90 degrees), significantly slowing down the accumulative rate, and the zigzag pattern for the dynamic of dew accumulation appeared.
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[Clinical observation on common peroneal nerve palsy treated with comprehensive therapy].
Zhongguo Zhen Jiu
PUBLISHED: 06-21-2014
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To compare the difference of the clinical efficacy on common peroneal palsy between the comprehensive therapy of electroacupuncture, moxibustion and moving cupping method and western medication.
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The complete chloroplast genome sequence of the Phyllostachys sulphurea (Poaceae: Bambusoideae).
Mitochondrial DNA
PUBLISHED: 06-19-2014
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Abstract The complete nucleotide sequence of the Phyllostachys sulphurea chloroplast genome (cpDNA) was determined in this study. The cpDNA was 139,731?bp in length, containing a pair of 21,798?bp inverted repeat regions (IR), which were separated by small and large single copy regions (SSC and LSC) of 12,879 and 83,256?bp, respectively. The P. sulphurea cp genome encodes 129 predicted functional genes; 110 are unique (77 protein-coding genes, 29 tRNA genes, 4 rRNA), 19 are duplicated in the IR regions and one gene extended into the IR region in the junctions between IR and SSC. 43.06% of the genome sequence encodes proteins. The P. sulphurea cp genome is AT-rich (61.11%). In these genes, fourteen genes contained one intron, while one gene had two introns.
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The complete chloroplast genome sequence of the Bambusa multiplex (Poaceae: Bambusoideae).
Mitochondrial DNA
PUBLISHED: 06-19-2014
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Abstract The complete nucleotide sequence of the Bambusa multiplex chloroplast genome (cpDNA) was determined in this study. The cpDNA was 139,394?bp in length, containing a pair of 21,798?bp inverted repeat regions (IR), which were separated by small and large single copy regions (SSC and LSC) of 12,875 and 82,923?bp, respectively. The B. multiplex cp genome encodes 129 predicted functional genes; 110 are unique (77 protein-coding genes, 29 tRNA genes, 4 rRNA), 19 are duplicated in the IR regions and one gene extended into the IR region in the junctions between IR and SSC. 43.20% of the genome sequence encodes proteins. The B. multiplex cp genome is AT-rich (61.08%). In these genes, fourteen genes contained one intron, while one gene had two introns.
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The complete chloroplast genome sequence of American bird pepper (Capsicum annuum var. glabriusculum).
Mitochondrial DNA
PUBLISHED: 05-10-2014
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Abstract The complete chloroplast genome sequence of American bird pepper (Capsicum annuum var. glabriusculum) is reported and characterized in this study. The genome size is 156,612?bp, containing a pair of inverted repeats (IRs) of 25,776?bp separated by a large single-copy region of 87,213?bp and a small single-copy region of 17,851?bp. The chloroplast genome harbors 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes, and 37 tRNA genes. A total of 18 of these genes are duplicated in the inverted repeat regions, 16 genes contain 1 intron, and 2 genes and one ycf have 2 introns.
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The complete chloroplast genome sequence of desert poplar (Populus euphratica).
Mitochondrial DNA
PUBLISHED: 05-10-2014
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Abstract The complete chloroplast sequence of the desert poplar (Populus euphratica), a plant well-adapted to salt stress, was determined in this study. The genome consists of 156,766?bp containing a pair of inverted repeats (IRs) of 16,591?bp separated by a large single-copy region and a small single-copy region of 84,888?bp and 27,646?bp, respectively. The chloroplast genome contains 130 known genes, including 89 protein-coding genes, 8 ribosomal RNA genes, and 37 tRNA genes; 18 of these are located in the inverted repeat region.
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[Effects of deep plowing and mulch in fallow period on soil water and yield of wheat in dryland].
Ying Yong Sheng Tai Xue Bao
PUBLISHED: 04-29-2014
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A field test was carried out in Qiujialing Village, Wenxi, Shanxi from 2009 to 2011 to study the soil water movement of 0-300 cm layer, yield formation and water use efficiency (WUE) of wheat with deep plowing and mulching the whole ground immediately (no mulch as control) 15 days and 45 days after harvest. The results indicated that deep plowing and mulch in fallow period could improve soil water storage of the 100-180 cm layer before sowing, the soil water storage efficiency in fallow period, and soil water storage from pre-wintering stage to booting stage. Compared with deep plowing 15 days after wheat harvest, deep plowing 45 days after wheat harvest did better in improving soil water storage and water use efficiency, as well as ear number and yield, which was more conducive in the year with more precipitation. Generally, deep plowing and mulching after raining during fallow period could benefit the soil water storage and conservation, thus would be helpful to improve wheat yield in dryland.
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Lateral patellar translation effects after arthroscopic partial meniscectomy of torn discoid lateral meniscus.
Orthop Surg
PUBLISHED: 03-05-2014
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To investigate the effects of arthroscopic partial meniscectomy of torn discoid lateral meniscus on patellar tracking.
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Pharmacokinetics of oxycodone hydrochloride and three of its metabolites after intravenous administration in Chinese patients with pain.
Pharmacol Rep
PUBLISHED: 02-01-2014
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The aim of this study is to evaluate the pharmacokinetic profile of oxycodone and three of its metabolites, noroxycodone, oxymorphone and noroxymorphone after intravenous administration in Chinese patients with pain.
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Mitochondrial genome of the black flying fox, Pteropus alecto (Chiroptera: Megachiroptera: Pteropodidae).
Mitochondrial DNA
PUBLISHED: 01-21-2014
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Abstract In this article we report the complete mitochondrial genome of black flying fox, Pteropus alecto, with the sequence length of 16,739 bp for the first time. The mitogenome contained a total of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and 1 control region. The base composition was A (33.1%), G (14.5%), C (27.2%) and T (25.2%), indicating that the percentage of A and T (58.3%) was higher than that of G and C. Most of these genes were distributed on the H-strand, except for the ND6 subunit gene and eight tRNA genes. The mitochondrial genome analyzed here will provide new genetic information to study the evolution of bats.
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The complete mitochondrial genome of western painted turtle, Chrysemys picta bellii (Chrysemys, Emydidae).
Mitochondrial DNA
PUBLISHED: 01-21-2014
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Abstract In this study, we report the complete mitochondrial genome sequence of western painted turtle, Chrysemys picta bellii. The genome is found to be 16,875?bp in length and has a base composition of A (34.4%), G (13.0%), C (26.0%), and T (26.6%). Similar to other turtles, it contains a typically conserved structure including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region (D-loop). Most of the genes are encoded on H-strand, except for the eight tRNA and ND6 genes. All protein-coding genes start with an ATN codon except for COX1 and ND4, which initiate with GTG instead, and terminate with the typical stop codon (TAA/TAG) or a single T (T-) or an unexpected codon of AGG. The complete mitochondrial genome sequence provided here would be useful for further phylogenetic analysis and conservation genetic studies in C. p. bellii.
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Preliminary detection of the anti?tumour activity of indoline?2,3?dione derivative DH?12a targeting aminopeptidase N.
Mol Med Rep
PUBLISHED: 01-09-2014
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Aminopeptidase N (APN) is important in tumour processes. The present study detected the anti?tumour activity of the novel APN inhibitor DH?12a, which is an indoline?2,3?dione derivative. In the present study, Bestatin, a clinical APN inhibitor was used as a positive control. The expression of APN in the ES-2 and 3AO cell lines were assessed using flow cytometry and the drug inhibition constants of DH?12a (Ki=13.15 µM) and Bestatin (Ki=16.57 µM) were assessed using a double reciprocal method of competitive inhibition. The in vitro effects of DH?12a on cell proliferation were assessed using a 3?(4,5?dimethyl?thiazol?2?yl)?2,5?diphenyl tetrazolium bromide assay on human cell lines of ES?2 (IC50=43.8 µM), A549 (inhibition rate=41.5% at 160 µM DH?12a), HL60 (inhibition rate=47.83% at 160 µM DH?12a) and 3AO (IC50=70.2 µM). The inhibition rates were consistently higher than those of Bestatin. The effects of DH?12a on cell migration (inhibition rates in ES?2 cells and 3AO cells were 56.4 and 76.5%, respectively at 15 µM) and invasion (inhibition rates in ES?2 cells and 3AO cells were 75.6 and 66.5%, respectively at 15 µM) were assessed using transwell plates. The in vivo effects of DH?12a on tumour proliferation and lung tumour metastasis were determined using an H22 xenograft mice model, where DH?12a was administered in combination with genotoxic 5?fluorouracil. The anti?tumour activities of DH?12a in vivo were also greater than those of Bestatin. In conclusion, the in vitro effects of DH?12a on tumour proliferation, migration and invasion were consistent with the in vivo effects. In addition, DH?12a exhibited greater anti?tumour properties compared with Bestatin.
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The predictive performance and stability of six species distribution models.
PLoS ONE
PUBLISHED: 01-01-2014
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Predicting species' potential geographical range by species distribution models (SDMs) is central to understand their ecological requirements. However, the effects of using different modeling techniques need further investigation. In order to improve the prediction effect, we need to assess the predictive performance and stability of different SDMs.
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Thirteen Camellia chloroplast genome sequences determined by high-throughput sequencing: genome structure and phylogenetic relationships.
BMC Evol. Biol.
PUBLISHED: 01-01-2014
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Camellia is an economically and phylogenetically important genus in the family Theaceae. Owing to numerous hybridization and polyploidization, it is taxonomically and phylogenetically ranked as one of the most challengingly difficult taxa in plants. Sequence comparisons of chloroplast (cp) genomes are of great interest to provide a robust evidence for taxonomic studies, species identification and understanding mechanisms that underlie the evolution of the Camellia species.
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Expansion of myeloid-derived suppressor cells from peripheral blood decreases after 4-week antiviral treatment in patients with chronic hepatitis C.
Int J Clin Exp Med
PUBLISHED: 01-01-2014
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Myeloid-derived suppressor cells (MDSCs) are one of the most important regulators of anti-tumor T-cell responses in cancers. This study aimed to investigate MDSCs in the peripheral blood of patients with chronic hepatitis C (CHC) before and after 4-week treatment with pegylated interferon (PEG-IFN) and ribavirin, and to evaluate their correlation with CD4(+)CD25(high) regulatory T cells (Tregs) and clinical parameters. A total of 80 patients with CHC were enrolled into this study, 37 of whom were treated with PEG-IFN and ribavirin. Compared with healthy controls (0.462% [range 0.257%-0.634%]), the proportion of MDSCs in the peripheral blood of 80 CHC patients (0.601% [range 0.333%-1.027%]) increased significantly before therapy (P=0.011). For 37 HCV patients, the proportion of circulating MDSCs (0 w: 0.597% [range 0.296%-1.021%], 4 w: 0.126% [0.066%-0.239%], P<0.01) and Tregs (0 w: 2.467±0.927%, 4 w: 2.074±0.840%, P=0.047) decreased significantly after 4-week antiviral treatment. No significant correlation was found between MDSCs and Tregs. These findings suggest that MDSCs expand in the peripheral blood of CHC patients, but decrease after 4-week antiviral treatment.
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[Effect of Midazolam on Mantle Cell Lymphoma JeKo-1 Cell Line and Its Relevant Mechanisms].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
PUBLISHED: 12-28-2013
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This study was aimed to explore the effect of midazolam on mantle cell lymphoma cell line JeKo-1 and the relevant mechanisms. Effects of midazolam on the proliferation and apoptosis of JeKo-1 cells were observed by CCK8 assay and flow cytometry, respectively. Effect of midazolam on the expression of BCL-2, cytochrome C(Cyto-C), pro-caspase-9, pro-caspase-8 and pro-caspase-3 protein were detected by Western blot. The results showed that midazolam could inhibit the growth of JeKo-1 cells significantly and the concentration of 50% growth inhibition (IC50) at 48 hours was approximately 40 µmol/L. After treatment with 20, 40, 80 µmol/L midazolam for 48 hours, a dose-dependent apoptosis of JeKo-1 cells could be observed. Meanwhile, a dose-dependent reduction of BCL-2, pro-caspase-9 and pro-caspase-3 protein expression and increase of Cyto-C protein expression in JeKo-1 cells were found, but the expression of pro-caspase-8 protein did not change. It is concluded that midazolam possibly initiates the mitochondrial pathway, not the death receptor pathway, by reducing the expression of BCL-2, leading in turn to the releasing of Cyto-C in mitochondria, then activating caspase-9 and caspase-3 protein, triggers the caspase cascade, and induces the apoptosis of JeKo-1 cells ultimately.
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[Up-regulator of cell proliferation predicts poor prognosis in hepatocellular carcinoma].
Zhonghua Yi Xue Za Zhi
PUBLISHED: 11-29-2013
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To explore the potential value of up-regulator of cell proliferation (URGCP) as a biomarker for predicting the prognosis of hepatocellular carcinoma (HCC).
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One-Step to Prepare Self-Organized Nanoporous NiO/TiO2 Layers and its Use in Non-Enzymatic Glucose Sensing.
Sci Rep
PUBLISHED: 10-29-2013
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A highly ordered nanoporous NiTi oxide layers were fabricated on Ti alloys with high Ni contents (50.6?at.%) by a combination of self-organizing anodization at 0°C and subsequent selective etching in H2O2. The key for successful formation of such layers is to sufficiently suppress the dissolve of NiO by applying lower temperature during anodization. The resulting nanoporous structure is connected and well-adhered, which exhibits a much higher electrochemical cycling stability in 0.1?M NaOH. Without further surface modification or the use of polymer binders, the layers can be behave as a low-cost, stable and sensitive platform in non-enzymatic glucose sensing.
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[Design of a compact structure interferometer].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 10-29-2013
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A novel interferometer system based on the combinations of cube-corner reflectors and fixed plane mirrors was designed, the moving mirror drive system was designed and analysed, and its governor PID algorithm was used to ensure that the movement of the moving mirror is collimated, uniform and smooth. The parameters of the optical system of the interferometer and the optical devices were described. Finally, after validation of the experiment, it was indicated that the wave number accuracy, resolution, signal to noise ratio and other key indicators can meet the needs of practical application.
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[Hepatitis B virus X promotes HepG2 cell cycle progression and growth via downregulation expression of p16 protein].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 10-15-2013
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To investigate the effects and related mechanisms of hepatitis B virus X (HBx) protein on cell cycle and growth in hepatocellular carcinoma.
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[Dynamic change of hepatitis B surface antigen expression in chronic hepatitis B patients during the natural recovery course and the short-term antivirus treatment].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 10-15-2013
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To investigate the dynamic quantitative changes in expression of hepatitis B virus (HBV) surface antigen (HBsAg) that occurs during the natural recovery course and the short-term antivirus treatment period of patients suffering from flares in chronic hepatitis B (CHB).
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[Intratympanic versus systemic steroid treatment for idiopathic sudden hearing loss: a meta-analysis].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-11-2013
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To assess the effectiveness and safety of intratympanic versus systemic steroid therapy in the initial treatment of idiopathic sudden hearing loss.
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Durability of efficacy after telbivudine off-treatment in chronic hepatitis B patients.
J. Clin. Virol.
PUBLISHED: 09-03-2013
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Current international guidelines indicate that finite therapy with nucleos(t)ide analogues (NAs) is possible in chronic hepatitis B (CHB) patients. Here we evaluate the durability of efficacy after telbivudine (LdT) off-treatment.
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Endothelial Insulin-Like Growth Factor-1 Modulates Proliferation and Phenotype of Smooth Muscle Cells Induced by Low Shear Stress.
Ann Biomed Eng
PUBLISHED: 08-31-2013
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Endothelial cells (ECs) are directly exposed to shear stress and modulate the neighboring vascular smooth muscle cells (VSMCs), which plays important roles in vascular remodeling during atherosclerosis. Our previous research revealed that insulin-like growth factors (IGFs) might participate in low shear stress (LowSS) induced vascular remodeling, which remains to be elucidated. Using EC/VSMC co-cultured parallel-plate flow chamber, LowSS (5 dyn/cm(2)) was applied and normal shear stress (NSS, 15 dyn/cm(2)) was used as control. LowSS induced IGF-1 secretion from ECs, which subsequently phosphorylated IGF-1 receptor (IGF-1R) on co-cultured VSMCs, then increased Akt phosphorylation and Sirt2 expression. Decreasing IGF-1 in ECs by RNA interference (RNAi) reversed these effects on VSMCs. Exogenous IGF-1 increased IGF-1R and Akt phosphorylation, Sirt2 expression, and proliferation of VSMCs, and induced VSMCs towards synthetic phenotype. PI3 K/Akt specific inhibitor wortmannin decreased Sirt2 expression, proliferation, and synthetic phenotype transformation of VSMCs, but had no effect on IGF-1R. Sirt2 RNAi repressed VSMC proliferation and phenotypic transformation, but had no effect on IGF-1R and Akt. Taken together, LowSS induces the secretion of IGF-1 from ECs, which subsequently paracrine influences the co-cultured VSMCs via IGF-1R and Akt phosphorylation, and Sirt2 expression, then results in the proliferation and synthetic phenotype transformation.
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Imbalance of interleukin-17-producing CD4 T cells/regulatory T cells axis occurs in remission stage of patients with hepatitis B virus-related acute-on-chronic liver failure.
J. Gastroenterol. Hepatol.
PUBLISHED: 08-27-2013
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Although regulatory T cells (Treg) and interleukin-17-producing CD4 T cells (Th17) have been demonstrated to play opposing roles in inflammation-associated diseases, their frequency and balance in different stages of hepatitis B virus (HBV)-related acute-on-chronic liver failure (ACLF) remain unknown.
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Phylogenetic relationships and genome divergence among the AA- genome species of the genus Oryza as revealed by 53 nuclear genes and 16 intergenic regions.
Mol. Phylogenet. Evol.
PUBLISHED: 08-17-2013
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Rapid radiations have long been regarded as the most challenging issue for elucidating poorly resolved phylogenies in evolutionary biology. The eight diploid AA- genome species in the genus Oryza represent a typical example of a closely spaced series of recent speciation events in plants. However, questions regarding when and how they diversified have long been an issue of extensive interest but remain a mystery. Here, a data set comprising >60kb of 53 singleton fragments and 16 intergenic regions is used to perform phylogenomic analyses of all eight AA- genome species plus four diploid Oryza species with BB-, CC-, EE- and GG- genomes. We fully reconstruct phylogenetic relationships of AA- genome species with confidence. Oryza meridionalis, native to Australia, is found to be the earliest divergent lineage around 2.93mya, whereas O. punctata, a BB- genome species, serves as the best outgroup to distinguish their phylogenetic relationships. They separated from O. punctata approximately 9.11mya during the Miocene epoch, and subsequently radiated to generate the entire AA- genome lineage diversity. The success in resolving the phylogeny of AA- genome species highlights the potential of phylogenomics to determine their divergence and evolutionary histories.
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[Design of high-efficiency double compound parabolic concentrator system in near infrared noninvasive biochemical analysis].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 08-03-2013
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High signal-to-noise ratio (SNR) of system is necessary to obtain accurate blood components in near infrared noninvasive biochemical analysis. In order to improve SNR of analytical system, high-efficiency double compound parabolic concentrator (DCPC) system was researched, which was aimed at increasing light utilization efficiency. Firstly, with the request of collection efficiency in near infrared noninvasive biochemical analysis, the characteristic of emergent rays through compound parabolic concentrator (CPC) was analyzed. Then the maximum focusing angle range of the first stage CPC was determined. Secondly, the light utilization efficiency of truncated type was compared with standard DCPC, thus the best structure parameters of DCPC system were optimized. Lastly, combined with optical parameters of skin tissue, calculations were operated when incident wavelength is 1 000 nm. The light utilization efficiency of DCPC system, CPC-focusing mirror system, and non-optical collecting system was calculated. The results show that the light utilization efficiency of the three optical systems is 1.46%, 0.84% and 0.26% respectively. So DCPC system enhances collecting ability for human diffuse reflection light, and helps improve SNR of noninvasive biochemical analysis system and overall analysis accuracy effectively.
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[Clinical analysis of 14 patients with extraorbital inflammatory myofibroblastic tumor of the head and neck].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 07-27-2013
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To summarize the clinical features of extraorbital inflammatory myofibroblastic tumor (IMT) of the head and neck.
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High level of IL-27 positively correlated with Th17 cells may indicate liver injury in patients infected with HBV.
Liver Int.
PUBLISHED: 06-20-2013
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Interleukin-6/IL-12 family cytokines play a key role in inflammatory diseases via their effects on the differentiation or regulation of T helper cells.
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[Effects of shuyu ningxln recipe on the praxiology and the expressions of hilppocampal BDNF and trkB of model rats with chronic stress-induced depression].
Zhongguo Zhong Xi Yi Jie He Za Zhi
PUBLISHED: 05-30-2013
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To observe the effects of Shuyu Ningxin Recipe (SNR) on the praxiology and the expressions of hippocampal brain-derived neurotrophic factor (BDNF) and its receptor tyrosine kinase B (TrkB) of model rats with chronic stress-induced depression, thus exploring its anti-depression mechanisms.
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Biotemplated synthesis of Au nanoparticles-TiO2 nanotube junctions for enhanced direct electrochemistry of heme proteins.
Chem. Commun. (Camb.)
PUBLISHED: 05-29-2013
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Au nanoparticles (AuNPs) are assembled on TiO(2) nanotube (TiNT) arrays by combining a biotemplating method with the unique photocatalytic properties of TiO(2). The formed AuNPs-TiNT junctions show highly improved electrochemical conductivity and act as electrode materials to achieve the enhanced direct electrochemistry of heme proteins.
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[Study on the application of empirical mode decomposition to noninvasive hemoglobin measurement by NIRS].
Guang Pu Xue Yu Guang Pu Fen Xi
PUBLISHED: 05-24-2013
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To increase the signal-to-noise ratio (SNR) of human near infrared (NIR) spectra, so as to improve the stability and precision of calibration model, the empirical mode decomposition (EMD) method was applied. Eighty-one fingertip absorption curves were collected, with the corresponding clinical examination results obtained immediately. By means of outliers detection and removal, finally 78 samples were determined as the research objects. A three-layer back-propagation artificial neutron network (BP-ANN) model was established and worked for prediction. The results turned out that, through EMD method, the prediction correlation coefficient increased greatly from 0.74 to 0.87. RMSEP was reduced from 12.85 to 8.08 g x L(-1). Other indexes were also obviously improved. The overall results sufficiently demonstrate that it is feasible to use EMD method forhigh SNR pulse wave signals, thus improving the performance of noninvasive hemoglobin calibration models. The application of EMD method can help promote the development of noninvasive hemoglobin monitoring technology.
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Serum proteomics analysis and comparisons using iTRAQ in the progression of hepatitis B.
Exp Ther Med
PUBLISHED: 05-15-2013
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The aim of this study was to analyze the changes in serum protein levels in the progression of hepatitis B using isobaric tags for relative and absolute quantitation (iTRAQ) analysis, in addition to comparing the serum protein levels of patients with chronic hepatitis B (CHB), patients with hepatitis B virus-induced acute-on-chronic liver failure (HBV-induced ACLF) and normal individuals. Protein analysis was performed on 15 serum samples using iTRAQ. The study population included healthy controls (n=5), patients with CHB (n=5) and patients with HBV-induced ACLF (n=5). Western blotting was used to verify the results in an additional nine serum samples from healthy controls, patients with CHB and patients with HBV-induced ACLF (n=3, respectively). Using iTRAQ analysis, 16 different serum proteins with ?1.5-fold differences in expression levels were identified in the patients with CHB and ACLF compared with the healthy controls. Five of those proteins, C-reactive protein precursor, hemoglobin ? chain variant Hb S-Wake, apolipoprotein J precursor, platelet factor 4 precursor and vitronectin, which demonstrated the greatest differences in their expression levels and the most significant correlation with liver diseases, were subsequently verified using western blotting. The western blotting results were consistent with the results from the iTRAQ. Two of the five proteins are not classified by biological process, and the biological functions of all the proteins in HBV-induced ACLF remain unclear. This preliminary study demonstrated that a correlation between the expression of various serum proteins and the different pathogenetic conditions induced by HBV may exist. The analysis of a larger number of samples is required to identify potential protein biomarkers that may be involved in the pathogenesis and progression of hepatitis B.
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[Quality of life survey on patients with peripheral facial paralysis by using Chinese version of the FaCE scale].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 05-10-2013
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To explore the health related quality of life (QOL) status of patients with peripheral facial paralysis.
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Nickel Hydroxide Nanoparticle Activated Semi-metallic TiO2 Nanotube Arrays for Non-enzymatic Glucose Sensing.
Chemistry
PUBLISHED: 04-19-2013
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Semi-metallic TiO2 nanotube arrays (TiOx Cy NTs) have been decorated uniformly with Ni(OH)2 nanoparticles without the aid of a polymer binder. The resulting hybrid nanotube arrays exhibit excellent catalytic activity towards non-enzymatic glucose electro-oxidation. The anodic current density of the glucose oxidation is significantly improved compared with traditional TiO2 nanotubes decorated with Ni(OH)2 . Moreover, the Ni(OH)2 /TiOx Cy NT-based electrode shows a fast response, high sensitivity, wide linear range, good selectivity and stability towards glucose electro-oxidation, and thus provides a promising and cost-effective sensing platform for non-enzymatic glucose detection.
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The human leukocyte antigen G promotes trophoblast fusion and ?-hCG production through the Erk1/2 pathway in human choriocarcinoma cell lines.
Biochem. Biophys. Res. Commun.
PUBLISHED: 04-01-2013
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The human leukocyte antigen G (HLA-G) is expressed on the fetal-maternal interface and plays a role in protecting fetal-derived trophoblasts from the maternal immune response, allowing trophoblasts to invade the uterus. However, HLA-G also possesses immune suppressing-independent functions. We found that HLA-G expressing BeWo choriocarcinoma cells increased cell-cell fusion compared to control BeWo cells under forskolin treatment. Regardless of forskolin treatment, the expression of fusogenic gene mRNAs, including syncytin-1, the transcription factor glial cell missing 1 (Gcm1), and beta human chorionic gonadotropin (?-hCG) were elevated. HLA-G up-regulates ?-hCG production in human choriocarcinoma cells because HLA-G knockdown in JEG-3 cells induces a dramatic decrease in ?-hCG compared with control cells. The defect in ?-hCG production in HLA-G knocked-down cells could not be completely overcome by stimulating hCG production through increasing intracellular cAMP levels. HLA-G expressing cells have increased phosphorylation levels for extracellular signal-regulated kinase1/2 (Erk1/2) in BeWo cells. The Erk1/2 pathway is inactivated after the inhibition of HLA-G expression in JEG-3 cells. Finally, Erk1/2 inhibition was able to suppress the increased hCG production induced by HLA-G expression. Together, these data suggest novel roles for HLA-G in regulating ?-hCG production via the modulation of the Erk1/2 pathway and by inducing trophoblast cell fusion.
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Molecular alterations of EGFR in small intestinal adenocarcinoma.
Int J Colorectal Dis
PUBLISHED: 03-18-2013
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Molecular testing for epidermal growth factor receptor (EGFR) mutations has recently become a standard practice for the management of patients with non-squamous none small cell lung cancer. Primary small intestine adenocarcinoma (SIA) is an uncommon malignancy, and EGFR mutation in the cancer has not been well characterized due to its rarity.
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[Fatigue and fracture resistance of the flared roots restored with computer aided design and computer aided manufacturing glass fiber posts].
Beijing Da Xue Xue Bao
PUBLISHED: 02-16-2013
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To evaluate the fatigue and fracture resistance of the flared roots restored with computer aided design (CAD) and computer aided manufacturing (CAM) glass fiber posts.
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Contradiction between plastid gene transcription and function due to complex posttranscriptional splicing: an exemplary study of ycf15 function and evolution in angiosperms.
PLoS ONE
PUBLISHED: 02-15-2013
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Plant chloroplast genes are usually co-transcribed while its posttranscriptional splicing is fairly complex and remains largely unsolved. On basis of sequencing the three complete Camellia (Theaceae) chloroplast genomes for the first time, we comprehensively analyzed the evolutionary patterns of ycf15, a plastid gene quite paradoxical in terms of its function and evolution, along the inferred angiosperm phylogeny. Although many species in separate lineages including the three species reported here contained an intact ycf15 gene in their chloroplast genomes, the phylogenetic mixture of both intact and obviously disabled ycf15 genes imply that they are all non-functional. Both intracellular gene transfer (IGT) and horizontal gene transfer (HGT) failed to explain such distributional anomalies. While, transcriptome analyses revealed that ycf15 was transcribed as precursor polycistronic transcript which contained ycf2, ycf15 and antisense trnL-CAA. The transcriptome assembly was surprisingly found to cover near the complete Camellia chloroplast genome. Many non-coding regions including pseudogenes were mapped by multiple transcripts, indicating the generality of pseudogene transcriptions. Our results suggest that plastid DNA posttranscriptional splicing may involve complex cleavage of non-functional genes.
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Prevalent role of gene features in determining evolutionary fates of whole-genome duplication duplicated genes in flowering plants.
Plant Physiol.
PUBLISHED: 02-08-2013
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The evolution of genes and genomes after polyploidization has been the subject of extensive studies in evolutionary biology and plant sciences. While a significant number of duplicated genes are rapidly removed during a process called fractionation, which operates after the whole-genome duplication (WGD), another considerable number of genes are retained preferentially, leading to the phenomenon of biased gene retention. However, the evolutionary mechanisms underlying gene retention after WGD remain largely unknown. Through genome-wide analyses of sequence and functional data, we comprehensively investigated the relationships between gene features and the retention probability of duplicated genes after WGDs in six plant genomes, Arabidopsis (Arabidopsis thaliana), poplar (Populus trichocarpa), soybean (Glycine max), rice (Oryza sativa), sorghum (Sorghum bicolor), and maize (Zea mays). The results showed that multiple gene features were correlated with the probability of gene retention. Using a logistic regression model based on principal component analysis, we resolved evolutionary rate, structural complexity, and GC3 content as the three major contributors to gene retention. Cluster analysis of these features further classified retained genes into three distinct groups in terms of gene features and evolutionary behaviors. Type I genes are more prone to be selected by dosage balance; type II genes are possibly subject to subfunctionalization; and type III genes may serve as potential targets for neofunctionalization. This study highlights that gene features are able to act jointly as primary forces when determining the retention and evolution of WGD-derived duplicated genes in flowering plants. These findings thus may help to provide a resolution to the debate on different evolutionary models of gene fates after WGDs.
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Well-oriented ZZ-PS-tag with high Fc-binding onto polystyrene surface for controlled immobilization of capture antibodies.
Anal. Chim. Acta
PUBLISHED: 01-04-2013
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The site specificity and bioactivity retention of antibodies immobilized on a solid substrate are crucial requirements for solid phase immunoassays. A fusion protein between an immunoglobulin G (IgG)-binding protein (ZZ protein) and a polystyrene-binding peptide (PS-tag) was constructed, and then used to develop a simple method for the oriented immobilization of the ZZ protein onto a PS support by the specific attachment of the PS-tag onto a hydrophilic PS. The orientation of intact IgG was achieved via the interaction of the ZZ protein and the constant fragment (Fc), thereby displayed the Fab fragment for binding antigen. The interaction between rabbit IgG anti-horseradish peroxidase (anti-HRP) and its binding partner HRP was analyzed. Results showed that the oriented ZZ-PS-tag yielded an IgG-binding activity that is fivefold higher than that produced by the passive immobilization of the ZZ protein. The advantage of the proposed immunoassay strategy was demonstrated through an enzyme-linked immunosorbent assay, in which monoclonal mouse anti-goat IgG and HRP-conjugated rabbit F(ab)2 anti-goat IgG were used to detect goat IgG. The ZZ-PS-tag presented a tenfold higher sensitivity and a wider linear range than did the passively immobilized ZZ protein. The proposed approach may be an attractive strategy for a broad range of applications involving the oriented immobilization of intact IgGs onto PS supports, in which only one type of phi-PS (ZZ-PS-tag) surface is used.
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Numerical simulation of airflow fields in two typical nasal structures of empty nose syndrome: a computational fluid dynamics study.
PLoS ONE
PUBLISHED: 01-01-2013
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The pathogenesis of empty nose syndrome (ENS) has not been elucidated so far. Though postulated, there remains a lack of experimental evidence about the roles of nasal aerodynamics on the development of ENS.
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Plastid genome sequence of a wild woody oil species, Prinsepia utilis, provides insights into evolutionary and mutational patterns of rosaceae chloroplast genomes.
PLoS ONE
PUBLISHED: 01-01-2013
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Prinsepiautilis Royle is a wild woody oil species of Rosaceae that yields edible oil which has been proved to possess particular benefits for human health and medical therapy. However, the lack of bred varieties has largely impeded exploiting immense potentials for high quality of its seed oil. It is urgently needed to enlarge the knowledge of genetic basis of the species and develop genetic markers to enhance modern breeding programs.
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Genome size variation among and within Camellia species by using flow cytometric analysis.
PLoS ONE
PUBLISHED: 01-01-2013
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The genus Camellia, belonging to the family Theaceae, is economically important group in flowering plants. Frequent interspecific hybridization together with polyploidization has made them become taxonomically "difficult taxa". The DNA content is often used to measure genome size variation and has largely advanced our understanding of plant evolution and genome variation. The goals of this study were to investigate patterns of interspecific and intraspecific variation of DNA contents and further explore genome size evolution in a phylogenetic context of the genus.
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[Quality of life in patients with chronic hepatitis C after PEG-Interferon a-2a therapy].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 12-29-2011
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To evaluate the quality of life (QOL) in the patients with chronic hepatitis C (CHC) after PEG-Interferon a-2a therapy.
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[Etiological surveillance and analysis of infectious diarrhea in Beijing in year 2010].
Zhonghua Yu Fang Yi Xue Za Zhi
PUBLISHED: 12-20-2011
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To explore the pathogenic form, epidemic features and serotype distribution of the pathogenic bacteria causing infectious diarrhea in Beijing.
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[Facial paralysis induced by herpes simplex virus type 1 and the study of facial neurons apoptosis in mice].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 12-20-2011
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To study the apoptosis of facial motor neurons and the expression of apoptosis-related genes, Bcl-2 and Bax, in the animal model of viral facial paralysis.
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[A five-year follow-up of one hundred and thirty-six patients of hepatitis C].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 12-01-2011
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To investigate the clinical outcome and effect of interferon treatment on patients with chronic hepatitis C.
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[Safety evaluation and quality control of MSCs from hepatitis B patient in vitro].
Zhonghua Gan Zang Bing Za Zhi
PUBLISHED: 11-05-2011
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To get mesenchymal stem cells (MSCs) from hepatitis B patient and to valuate the safety and quality after long-term culture in vitro.
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[Effect of acupuncture of "Zusanli"(ST 36)on sexual hormone levels in spleen-deficiency syndrome rats].
Zhen Ci Yan Jiu
PUBLISHED: 09-28-2011
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To study the underlying mechanism of acupuncture of "Zusanli" (ST 36) in clinical treatment of spleen deficiency syndrome (SDS).
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[Study of characteristic of the cochlear hydrops analysis masking procedure in normal adults].
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
PUBLISHED: 09-20-2011
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To study the characteristic of the cochlear hydrops analysis masking procedure (CHAMP) in normal adults, and to evaluate the diagnostic values of its parameters for membranous labyrinth hydrops.
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What is Visualize?

JoVE Visualize is a tool created to match the last 5 years of PubMed publications to methods in JoVE's video library.

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We use abstracts found on PubMed and match them to JoVE videos to create a list of 10 to 30 related methods videos.

Video X seems to be unrelated to Abstract Y...

In developing our video relationships, we compare around 5 million PubMed articles to our library of over 4,500 methods videos. In some cases the language used in the PubMed abstracts makes matching that content to a JoVE video difficult. In other cases, there happens not to be any content in our video library that is relevant to the topic of a given abstract. In these cases, our algorithms are trying their best to display videos with relevant content, which can sometimes result in matched videos with only a slight relation.