Live Cell Imaging of Lipid Droplet Size and Fusion Using Oil Red O and BODIPY

Summary

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The present protocol describes how to use oil red O to dye lipid droplets (LDs), calculate the size and number of LDs in a fatty acid-induced fatty hepatocyte model, and use BODIPY 493/503 to observe the process of small LDs fusing into large LDs by live cell imaging.