Semi-Automated Isolation of Murine White Adipose Tissue

May 19th, 2023

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator (Video) | JoVE

02:39 min

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Isolation, Digestion, Filtration and Cell Plating of Murine Adipose Tissue (Video) | JoVE

03:47 min

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Kaede Saito^1,2, Yuta Hiraike^1,2,3, Misato Oguchi², Toshimasa Yamauchi²

¹Division for Health Service Promotion, The University of Tokyo, ²Department of Diabetes and Metabolic Diseases, Graduate School of Medicine, The University of Tokyo, ³The University of Tokyo Excellent Young Researcher Program, The University of Tokyo

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Detta protokoll beskriver den halvautomatiska isoleringen av den stromala vaskulära fraktionen (SVF) från murin fettvävnad för att erhålla preadipocyter och uppnå adipocytdifferentiering in vitro. Användning av en vävnadsdissociator för kollagenassmältning minskar experimentell variation och ökar reproducerbarheten.

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Semi-Automated Isolation of Murine White Adipose Tissue

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