Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

High-Resolution 3D Reconstruction of Human Brain with SHORT and LSFM

January 26th, 2024
*1, *1, *1,2, 1, 1,3, 1, 1,4,5, 1,4,6, 1,4,5
1European Laboratory for Non-linear Spectroscopy (LENS), University of Florence, 2Division of Physiology, Department of Experimental and Clinical Medicine, University of Florence, 3Department of Physics, University of Pisa, 4National Research Council - National Institute of Optics (CNR-INO), 5Department of Physics and Astronomy, University of Florence, 6Department of Biology, University of Florence
* These authors contributed equally

Summary
Automatic Translation

Il presente protocollo fornisce una procedura passo-passo per la clearing ottica rapida e simultanea, la marcatura multi-round e la ricostruzione volumetrica 3D di decine di sezioni di cervello umano post-mortem combinando la tecnica di trasformazione tissutale corta (SWITCH - H2O2 - Antigen Retrieval - 2,2'-tiodiethanol [TDE]) SHORT con l'imaging al microscopio a fluorescenza a foglio luminoso in un protocollo di routine ad alto rendimento.

Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter