Guizhou Medical University 3 articles published in JoVE Biology Generating the Transcriptional Regulation View of Transcriptomic Features for Prediction Task and Dark Biomarker Detection on Small Datasets Kewei Li1, Yusi Fan1, Yaqing Liu1, Hongmei Liu2, Gongyou Zhang2, Meiyu Duan1, Lan Huang1, Fengfeng Zhou1 1College of Computer Science and Technology, and Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of Education, Jilin University, 2School of Biology and Engineering, Guizhou Medical University Here, we introduce a protocol for converting transcriptomic data into a mqTrans view, enabling the identification of dark biomarkers. While not differentially expressed in conventional transcriptomic analyses, these biomarkers exhibit differential expression in the mqTrans view. The approach serves as a complementary technique to traditional methods, unveiling previously overlooked biomarkers. Immunology and Infection Detecting Wolbachia Strain wAlbB in Aedes albopictus Cell Lines Lingling Chen*1,2, Qiuqiu Xiao*1, Mengting Shi1, Jinzhi Cheng1, Jiahong Wu1 1Guizhou Medical University, 2Guizhou Health Vocational College Four methods were used to detect intracellular Wolbachia, which complemented each other and improved the detection accuracy of Wolbachia infection of Aedes albopictus-derived Aa23 and Aa23-T cured of native Wolbachia infection using antibiotics. Neuroscience Auditory Brainstem Response and Outer Hair Cell Whole-cell Patch Clamp Recording in Postnatal Rats Aoshuang Chang1,2, Cuixian Li1, Jianfeng Huang1,3, Wenlu Pan1, Yinghong Tian4, Jie Tang1 1Department of Physiology, School of Basic Medical Sciences, Southern Medical University, 2School of Basic Medical Sciences, Guizhou Medical University, 3Department of Laboratory Medicines, Guangzhou General Hospital of Guangzhou Military Region, Southern Medical University, 4Experiment Teaching Center, School of Basic Medical Sciences, Southern Medical University This protocol describes methods to record the auditory brainstem response from postnatal rat pups. To examine the functional development of outer hair cells, the experimental procedure of whole-cell patch clamp recording in isolated outer hair cells is described step-by-step.