Xuzhou Medical University 4 articles published in JoVE Bioengineering Prospective, Randomized, and Controlled Study of a Human Umbilical Cord Mesenchymal Stem Cell Injection for Treating Diabetic Foot Ulcers Jingyu Zhang*1,2, Bingkun Zhao*1, Wuhan Wei*1,2, Dan Wang3,4,5, Haoyu Wang1,2, Aijun Zhang1, Changbo Tao1, Xueyang Li1, Qiang Li1, Peisheng Jin1 1Department of Plastic Surgery, Affiliated Hospital of Xuzhou Medical University, 2Graduate School, Xuzhou Medical University, 3Institute for Tissue Engineering and Regenerative Medicine, The Chinese University of Hong Kong, 4School of Biomedical Sciences, The Chinese University of Hong Kong, 5Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park The present protocol describes a prospective, randomized, controlled clinical study that evaluates a human umbilical cord mesenchymal stem cell injection for the treatment of chronic diabetic foot ulcers. Biology Structure-Based Simulation and Sampling of Transcription Factor Protein Movements along DNA from Atomic-Scale Stepping to Coarse-Grained Diffusion Chao E*1, Liqiang Dai*1,2, Jiaqi Tian3,4, Lin-Tai Da4, Jin Yu5,6,7 1Beijing Computational Science Research Center, 2Shenzhen JL Computational Science and Applied Research Institute, 3School of Medical Informatics and Engineering, Xuzhou Medical University, 4Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 5Department of Physics and Astronomy, University of California, Irvine, 6Department of Chemistry, University of California, Irvine, 7NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine The goal of this protocol is to reveal structural dynamics of one-dimensional diffusion of protein along DNA, using a plant transcription factor WRKY domain protein as an exemplary system. To do this, both atomistic and coarse-grained molecular dynamics simulations along with extensive computational samplings have been implemented. Biochemistry The Extraction of Liver Glycogen Molecules for Glycogen Structure Determination Ziyi Wang1,2, Qinghua Liu3,4, Liang Wang3,5, Robert G. Gilbert1,2,6, Mitchell A. Sullivan7 1School of Chemistry and Molecular Biosciences, The University of Queensland, 2Centre for Nutrition and Food Sciences, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, 3Jiangsu Provincial Key Laboratory of New Drug Research and Clinical Pharmacy, School of Pharmacy, Xuzhou Medical University, 4Department of Pharmaceutical Analysis, School of Pharmacy, Xuzhou Medical University, 5Department of Bioinformatics, School of Medical Informatics and Engineering, Xuzhou Medical University, 6Joint International Research Laboratory of Agriculture and Agri-Product Safety, College of Agriculture, Yangzhou University, 7Glycation and Diabetes Group, Mater Research Institute-The University of Queensland, Translational Research Institute An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial. Chemistry Methionine Functionalized Biocompatible Block Copolymers for Targeted Plasmid DNA Delivery Yang Wu*1, Wei Zhang*2, Jian Zhang2, Zhi-Xiang Mao3, Li Ding2, Hao Li3, Rong Ma1, Jin-Hai Tang2 1Research Center of Clinical Oncology, Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research & Nanjing Medical University Affiliated Cancer Hospital, 2Department of General Surgery, The First Affiliated Hospital with Nanjing Medical University, 3School of Clinical Medicine, Xuzhou Medical University This work presents the preparation of methionine functionalized biocompatible block copolymers (mBG) via the reversible addition-fragmentation chain transfer (RAFT) method. The plasmid DNA complexing ability of the obtained mBG and their transfection efficiency were also investigated. The RAFT method is very beneficial for polymerizing monomers containing special functional groups.