University Medical Center of the Johannes Gutenberg-University 12 articles published in JoVE Medicine Lipopolysaccharide Infusion as a Porcine Endotoxemic Shock Model Andrea Urmann1, Katja Mohnke1, Julian Riedel1, Johanna Hain1, Miriam Renz1, René Rissel1, Bastian Duenges1, Robert Ruemmler1, Alexander Ziebart1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University We provide a protocol for an experimental endotoxemic shock model in pigs by infusion of lipopolysaccharide. Medicine Endotracheal Intubation Using a Flexible Intubation Endoscope as a Standardized Model for Safe Airway Management in Swine Katja Mohnke1, Julian Riedel1, Miriam Renz1, René Rissel1, Alexander Ziebart1, Jens Kamuf1, Erik Kristoffer Hartmann1, Robert Ruemmler1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University The use of pigs in research has increased in recent years. Nevertheless, pigs are characterized by difficult airway anatomy. By demonstrating how to perform endoscopically guided endotracheal intubation, the present protocol aims to further increase laboratory animals' safety to avoid animal suffering and unnecessary death. Neuroscience Lipidomics and Transcriptomics in Neurological Diseases Julia M. Post1, Raissa Lerner1, Claudia Schwitter1, Beat Lutz1, Ermelinda Lomazzo1, Laura Bindila1 1Institute of Physiological Chemistry, University Medical Center of the Johannes Gutenberg University Mainz This article presents a modular protocol for tissue lipidomics and transcriptomics, and plasma lipidomics in neurological disease mouse models targeting lipids underlying inflammation and neuronal activity, membrane lipids, downstream messengers, and mRNA-encoding enzymes/receptors underlying lipid function. Sampling, sample processing, extraction, and quantification procedures are outlined. Neuroscience In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal Paul Schramm1, Florian Hetsch2, Jochen C. Meier3, Reinhard W. Köster1 1Cellular and Molecular Neurobiology, Zoological Institute, Technische Universität Braunschweig, 2Institute of Pathophysiology, University Medical Center of the Johannes Gutenberg University, 3Cell Physiology, Zoological institute, Technische Universität Braunschweig Here we present a method to image the zebrafish embryonic brain in vivo upto larval and juvenile stages. This microinvasive procedure, adapted from electrophysiological approaches, provides access to cellular and subcellular details of mature neuron and can be combined with optogenetics and neuropharmacological studies for characterizing brain function and drug intervention. Medicine Standardized Model of Ventricular Fibrillation and Advanced Cardiac Life Support in Swine Robert Ruemmler1, Alexander Ziebart1, Andreas Garcia-Bardon1, Jens Kamuf1, Erik K. Hartmann1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University Cardiopulmonary resuscitation and defibrillation are the only effective therapeutic options during cardiac arrest caused by ventricular fibrillation. This model presents a standardized regimen to induce, assess, and treat this physiological state in a porcine model, thus providing a clinical approach with various opportunities for data collection and analysis. Neuroscience Electrophysiological Investigations of Retinogeniculate and Corticogeniculate Synapse Function Xufeng Chen1, Danni Wang1, Marcel Kegel1, Jakob von Engelhardt1 1Institute of Pathophysiology, University Medical Center of the Johannes Gutenberg University Mainz Here, we present protocols for the preparation of acute brain slices containing the lateral geniculate nucleus and the electrophysiological investigation of retinogeniculate and corticogeniculate synapses function. This protocol provides an efficient way to study synapses with the high-release and low-release probability in the same acute brain slices. Medicine Standardized Hemorrhagic Shock Induction Guided by Cerebral Oximetry and Extended Hemodynamic Monitoring in Pigs Alexander Ziebart1, Jens Kamuf1, Robert Ruemmler1, Rene´ Rissel1, Moritz Gosling1, Andreas Garcia-Bardon1, Erik K. Hartmann1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University Hemorrhagic shock is a severe complication in seriously injured patients, which leads to life-threatening oxygen undersupply. We present a standardized method to induce hemorrhagic shock via blood withdrawal in pigs that is guided by hemodynamics and microcirculatory cerebral oxygenation. Biology Sample Preparation for Mass-spectrometry-based Proteomics Analysis of Ocular Microvessels Natarajan Perumal*1, Lars Straßburger1, Carsten Schmelter1, Adrian Gericke1, Norbert Pfeiffer1, Franz H. Grus1, Caroline Manicam*1 1Department of Ophthalmology, University Medical Centre of the Johannes Gutenberg University Mainz Proteome characterization of ocular microvascular beds is pivotal for in-depth understanding of many ocular pathologies in humans. This study demonstrates an effective, rapid, and robust method for protein extraction and sample preparation from small blood vessels employing the porcine short posterior ciliary arteries as model vessels for mass-spectrometry-based proteomics analyses. Medicine Oleic Acid-Injection in Pigs As a Model for Acute Respiratory Distress Syndrome Jens Kamuf1, Andreas Garcia-Bardon1, Alexander Ziebart1, Rainer Thomas1, Robert Rümmler1, Christian Möllmann1, Erik K. Hartmann1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University In this article, we present a protocol to induce acute lung injury in pigs by central-venous injection of oleic acid. This is an established animal model for studying the acute respiratory distress syndrome (ARDS). Neuroscience A Volumetric Method for Quantification of Cerebral Vasospasm in a Murine Model of Subarachnoid Hemorrhage Axel Neulen*1, Michael Kosterhon*1, Tobias Pantel*1, Stefanie Kirschner2, Hermann Goetz3, Marc A. Brockmann2, Sven R. Kantelhardt1, Serge C. Thal4 1Department of Neurosurgery, Medical Center of the Johannes Gutenberg - University, 2Department of Neuroradiology, Medical Center of the Johannes Gutenberg - University, 3Platform for Biomaterial Research, Medical Center of the Johannes Gutenberg - University, 4Department of Anesthesiology, Medical Center of the Johannes Gutenberg - University The goal of this article is to present a method that allows a 3-dimensional reconstruction of the cerebrovascular tree in mice after micro computed tomography and determination of volumes of entire vessel segments that can be used to quantify cerebral vasospasm in murine models of subarachnoid hemorrhage. Medicine Topical Airway Anesthesia for Awake-endoscopic Intubation Using the Spray-as-you-go Technique with High Oxygen Flow Nina Pirlich1, Jana A. Lohse1, Rüdiger R. Noppens1 1Department of Anesthesiology, University Medical Center of the Johannes Gutenberg-University The topical anesthetic lidocaine was atomized using a high oxygen flow through the working channel of a flexible intubating endoscope to achieve topical airway anesthesia for awake endotracheal intubation. We prefer this modified spray-as-you-go technique for endoscopic intubation to classical bolus application because of higher patient satisfaction and better compliance. Developmental Biology Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues Imen Jebeniani1, Julia Leschik2, Michel Puceat1 1INSERM UMR-910, Faculté de Médecine, Université Aix-Marseille, 2Institute of Physiological Chemistry, University Medical Center, Johannes Gutenberg University A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.
