Oslo University Hospital 7 articles published in JoVE Biology Rapid, Cost-Efficient, Enzyme-Free Passaging of Human Pluripotent Stem Cells on Feeder Cells by Ethylenediaminetetraacetic Acid-Mediated Dis-Adhesion Hege Brincker Fjerdingstad1, Joel C. Glover1,2 1Norwegian Center for Stem Cell Research, Department of Immunology and Transfusion Medicine, Oslo University Hospital, 2Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Molecular Medicine, Institute of Basic Medical Sciences, University of Oslo To avoid the limitations associated with the enzymatic or mechanical passaging of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) cultured on feeder cells, we have established a fast, effective, cost-efficient, high-yield method for harvesting hESC or hiPSC colonies maintained on a feeder cell layer of human foreskin fibroblasts using EDTA-mediated dis-adhesion. Medicine DIPLOMA Approach for Standardized Pathology Assessment of Distal Pancreatectomy Specimens Sanne Lof1, Rushda Rajak2, Frederique L. I. M. Vissers3, Maarten Korrel3, Adrian Bateman2, Johanna Verheij4, Caroline Verbeke5, Ivana Cataldo6, Marc G. Besselink3, Mohammed Abu Hilal1 1Department of Surgery, Southampton University Hospital NHS Foundation Trust, 2Department of Pathology, Southampton University Hospital NHS Foundation Trust, 3Department of Surgery, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 4Department of Pathology, Cancer Center Amsterdam, Amsterdam UMC, University of Amsterdam, 5Department of Pathology, Oslo University Hospital & University of Oslo, 6Department of Pathology, Ca Foncello Hospital The current study highlights a standardized approach to the macroscopic assessment of distal pancreatectomy specimens for pancreatic ductal adenocarcinoma, with special emphasis on the measurement of pancreatic dimensions and those of other organs, inking of margins, measurement of tumor size and proximity to margins, lymph node sampling and block selection. Cancer Research A Spheroid Killing Assay by CAR T Cells Pierre Dillard1, Hakan Köksal1, Else-Marit Inderberg1, Sebastien Wälchli1 1Department of Cellular Therapy, Department of Oncology, Oslo University Hospital-Radiumhospitalet This protocol is designed to assess immunotherapeutic redirected T-cell (CAR T-cell) cytotoxicity against 3D structured cancerous cells (spheroids) in real time. Neuroscience Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy Lauritz Hagen Kennedy1, Johanne Egge Rinholm1,2 1Division of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 2Department of Microbiology, Oslo University Hospital Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented. Medicine The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo Ane M. Holme1,2, Maia B. Holm1,2, Marie C. P. Roland1,3, Hildegunn Horne1,2, Trond M. Michelsen1,3, Guttorm Haugen2,4, Tore Henriksen1,2 1Department of Obstetrics, Oslo University Hospital, 2Institute of Clinical Medicine, University of Oslo, 3Norwegian Advisory Unit on Women's Health, Oslo University Hospital, 4Department of Fetal Medicine, Oslo University Hospital We present a detailed method to study human placental physiology in vivo at term. The method combines blood sampling from the incoming and outgoing vessels on the maternal and fetal sides of the placenta with ultrasound measurements of volume blood flow and placental tissue sampling. Immunology and Infection Flow Cytometry-based Assay for the Monitoring of NK Cell Functions Sara Tognarelli*1,2, Benedikt Jacobs*3,4, Nina Staiger1,2, Evelyn Ullrich1,2 1Childrens Hospital, Department of Pediatric Stem Cell Transplantation and Immunology, Johann Wolfgang Goethe-University, 2LOEWE Center for Cell and Gene Therapy, Johann Wolfgang Goethe-University, 3Institute for Cancer Research, Department of Cancer Immunology, Oslo University Hospital, Radiumhospital, 4The KG Jebsen Center for Cancer Immunotherapy, Institute of Clinical Medicine, University of Oslo A simple and reliable method is described here to analyze a set of NK cell functions such as degranulation, cytokine and chemokine production within different NK cell subsets. Medicine A Neonatal Mouse Spinal Cord Compression Injury Model Mark Züchner1,2, Joel C. Glover2,3, Jean-Luc Boulland2,3 1Department of Neurosurgery, Oslo University Hospital, 2Norwegian Center for Stem Cell Research, Oslo University Hospital, 3Laboratory of Neural Development and Optical Recording (NDEVOR), Department of Physiology, Institute of Basic Medical Sciences, University of Oslo This article describes a method for generating a reproducible spinal cord compression injury (SCI) in the neonatal mouse. The model provides an advantageous platform for studying mechanisms of adaptive plasticity that underlie spontaneous functional recovery.