University of Miami, Rosenstiel School of Marine and Atmospheric Sciences 1 article published in JoVE Environment In Situ Hybridization Techniques for Paraffin-Embedded Adult Coral Samples Nikki Traylor-Knowles1 1Department of Marine Biology and Ecology, University of Miami, Rosenstiel School of Marine and Atmospheric Sciences The goal of this protocol is to perform in situ hybridization on adult coral samples that have been embedded in paraffin and sectioned onto glass slides. This is a qualitative method used to visualize the spatial expression of an RNA anti-sense probe in paraffin-embedded tissues.