Encyclopedia of Experiments: Immunology
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Transcript
Natural killer, NK, cells are large granular lymphocytes that eliminate tumors and microbial infections.
For ex vivo NK cell expansion, take a suspension of peripheral blood mononuclear cells, PBMCs, containing a heterogenous population of lymphocytes, including NK cells. Add irradiated feeder cells — non-proliferating lymphoblastoid cells — which express membrane-bound interleukin 21, mIL-21, required to activate and expand NK cells.
Add the co-culture in a specialized culture well with a gas-permeable membrane at the bottom, allowing gas exchange. The cells settle down and proliferate for an extended period without requiring passaging.
Add interleukin 2, IL-2, and interleukin 15, IL-15 — cytokines for NK cell proliferation. Incubate for an adequate period in physiological conditions.
mIL-21 on the feeder cells binds to a specific heterodimeric receptor on the NK cells. Exogenous IL-15 and IL-2 bind to their respective receptors. The binding induces different downstream signaling pathways — activating gene expression — limiting cellular senescence and leading to prolonged NK cell proliferation.
Using flow cytometry, assess the NK cell expansion at regular time intervals.
Plot the recorded data to analyze the expansion rate of the cells and their purity — selective growth of the target NK cell type. The manifold expansion over the incubation period indicates a sustained proliferation while maintaining high purity.
