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Journal / Immunology and Infection / Imaging Neutrophils and Monocytes in Mesenteric…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
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Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time

Article DOI: 10.3791/53314-v • 09:28 min • November 16th, 2015
November 16th, 2015 •
1, 1, 1
1Department of Pathology and Immunology, University of Geneva

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Summary
Automatic Translation

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

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Intravital Microscopy Neutrophils Monocytes Mesenteric Veins Real-time Imaging Immune Response Leukocyte Migration Transendothelial Migration Vascular Endothelium Fluorescent Proteins Transgenic Mice Confocal Microscope Time-lapse Movies Steady State Inflammatory Conditions Blood Vessel Inflammation TLR2/TLR1 Agonist Pam3SK4 Leukocyte Recruitment Trafficking
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