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JoVE Journal Biochemistry
Aislamiento y medidas respiratoria de las mitocondrias de Arabidopsis thaliana
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Aislamiento y medidas respiratoria de las mitocondrias de Arabidopsis thaliana

Article DOI: 10.3791/56627-v • 09:54 min • January 5th, 2018
January 5th, 2018 •
1, 1, 1, 2, 3, 1, 1
1ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, 2School of Biological Sciences, Flinders University, 3ARC Centre of Excellence in Plant Energy Biology, University of Western Australia

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Como las mitocondrias son sólo un pequeño porcentaje de la célula de la planta, tienen que ser purificado para una gama de estudios. Las mitocondrias pueden ser aisladas de una variedad de órganos de la planta de homogeneización, seguido de centrifugación gradiente diferencial y densidad para obtener una fracción altamente purificada mitocondrial.

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Bioquímica número 131 Arabidopsis thaliana aislamiento mitocondrial gradientes de densidad pureza fraccionamiento mediciones respiratorias electroforesis en gel de poliacrilamida nativo azul (BN-PAGE) sodio dodecil sulfato-poliacrilamida gel de electroforesis (SDS-PAGE) western blot
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