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Journal / Biochemistry / 单核苷酸延长和 MALDI 质谱分析校对及 DNA…
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Biochemistry

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JoVE Journal Biochemistry
单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
Journal (Biochemistry)
Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins
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单核苷酸延长和 MALDI 质谱分析校对及 DNA 修复方法

Article DOI: 10.3791/57862-v • 11:08 min • June 19th, 2018
June 19th, 2018 •
*1,2, *3, 1, 1, 1, 2, 1,2, 1,2, 1,2
1Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, 2Department of Laboratory Medicine, National Taiwan University Hospital, 3Center for Microbial Pathogenesis, Nationwide Children's Hospital and the Department of Pediatrics, The Ohio State University
* These authors contributed equally

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采用高分辨率 MALDI 质谱和单核苷酸扩展策略, 建立了 dna 聚合酶校对和 dna 修复实验的非标记非射电同位素方法。试验证明是非常具体, 简单, 快速, 并易于执行校对和修复补丁短于 9-核苷酸。

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生物化学 问题 136 dna 聚合酶校对 dna 修复 MALDI 的质谱 单核苷酸扩展 dideoxyribonucleotide 三磷酸 dna 不匹配 dna 复制错误
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