JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
Here, we present a protocol to detect total cellular reactive oxygen species (ROS) using 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). This method can visualize cellular ROS localization in adherent cells with a fluorescence microscope and quantify ROS intensity with a fluorescence plate reader. This protocol is simple, efficient and cost-effective.