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Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells
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Quantifying Spatiotemporal Parameters of Cellular Exocytosis in Micropatterned Cells

Article DOI: 10.3791/60801-v • 10:21 min • September 16th, 2020
September 16th, 2020 •
1,2,3, 1,2,3, 4, 1,2,3
1Unité Mixte de Recherche 144 CNRS, Molecular Mechanisms of Intracellular Transport group, Institut Curie, 75005 Paris, France, 2PSL Research University, Paris, France, 3Sorbonne Université, Paris, France, 4INRIA, Université Paris-Sud, PSL

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Summary
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Live imaging of lysosomal exocytosis on micropatterned cells allows a spatial quantification of this process. Morphology normalization using micropatterns is an outstanding tool to uncover general rules about the spatial distribution of cellular processes.

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Quantifying Spatiotemporal Parameters Cellular Exocytosis Micropatterned Cells Live Imaging TOF Microscopy Clustering Events Regions Of High Activity Cell Cultures Micropattern Surfaces Cell Division Secretory Events Macromolecules Immune Regulation Cancer Proteolytic Enzymes Invasion Quantification Of Secretion Degraded Secretion Dynamic Processes Antigen Presentation Imaging Protocol Analysis Protocol Single Cells Micropatterning Coverslips Polylysine Graft Polyethylene Glycol
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