JoVE Journal
Biology
Biology
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Summary
Please note that all translations are automatically generated.
该方案说明了1)从成年小鼠腓肠肌中分离和培养原代成纤维细胞,以及2)使用差异超速离心法结合蔗糖密度梯度,然后进行蛋白质印迹分析的外泌体纯化和表征。