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Biochemistry

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JoVE Journal Biochemistry
MS2-Affinitätsreinigung gekoppelt mit RNA-Sequenzierung in grampositiven Bakterien
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MS2-Affinitätsreinigung gekoppelt mit RNA-Sequenzierung in grampositiven Bakterien

Article DOI: 10.3791/61731-v • 08:34 min • February 23rd, 2021
February 23rd, 2021 •
1, 2, 2, 1, 1, 1
1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke

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Die MAPS-Technologie wurde entwickelt, um das Targetom einer bestimmten regulatorischen RNA in vivo zu untersuchen. Die sRNA von Interesse ist mit einem MS2-Aptamer gekennzeichnet, das die Co-Reinigung ihrer RNA-Partner und deren Identifizierung durch RNA-Sequenzierung ermöglicht. Dieses modifizierte Protokoll eignet sich besonders für grampositive Bakterien.

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Retraction MAPS Regulatory RNA RNA sequencing Targetome Regulatory network Co-purification MS2 aptamer Gram-positive bacteria Staphylococcus aureus
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