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Neuroscience

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JoVE Journal Neuroscience
In vitro kwantitatieve beeldvormingstest voor fagocytose van dode neuroblastoomcellen door iPSC-macrofagen
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In vitro kwantitatieve beeldvormingstest voor fagocytose van dode neuroblastoomcellen door iPSC-macrofagen

Article DOI: 10.3791/62217-v • 09:48 min • February 14th, 2021
February 14th, 2021 •
1,2,3, 2, 1, 2, 1
1James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, 2Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building, University of Oxford, 3UK Dementia Research Institute, Cardiff University

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Neurodegeneratieve ziekten worden geassocieerd met ontregelde microgliafuncties. Dit artikel schetst een in vitro test van fagocytose van neuroblastoomcellen door iPSC-macrofagen. Kwantitatieve microscopie-uitleingen worden beschreven voor zowel live-cell time-lapse imaging als fixed-cell high-content imaging.

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Neurowetenschappen fagocytose efferocytose iPSC-macrofaag microglia neuron SH-SY5Y high-content live-cell time-lapse neurodegeneratie apoptotisch
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