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Detecção de glicosaminoglicanos por Electrophoresis gel poliacrilamida e coloração de prata
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Detecção de glicosaminoglicanos por Electrophoresis gel poliacrilamida e coloração de prata

Article DOI: 10.3791/62319-v • 05:57 min • February 25th, 2021
February 25th, 2021 •
1,2, 3,4, 1, 1, 3, 1,5
1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center

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Este relatório descreve técnicas para isolar e purificar glicosaminoglycanos sulfatos (GAGs) de amostras biológicas e uma abordagem de eletroforese de gel de poliacrilamida para aproximar seu tamanho. Os GAGs contribuem para a estrutura tecidual e influenciam os processos de sinalização através da interação eletrostática com proteínas. O comprimento do polímero GAG contribui para sua afinidade vinculante para ligantes cognatos.

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Biologia Edição 168
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