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Bereiding van ratskeletspieren homogenaten voor nitraat- en nitrietmetingen
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Bereiding van ratskeletspieren homogenaten voor nitraat- en nitrietmetingen

Article DOI: 10.3791/62427-v • 07:19 min • July 29th, 2021
July 29th, 2021 •
*1, *1, 2, 2, 2, 1, 1
1Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 2Sport and Health Sciences, College of Life and Environmental Sciences, St Luke’s Campus, University of Exeter
* These authors contributed equally

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We presenteren protocollen voor drie verschillende methoden voor de homogenisatie van vier verschillende spiergroepen van rattenskeletspierweefsel om de niveaus van nitraat en nitriet te meten en te vergelijken. Verder vergelijken we verschillende monstergewichten om te onderzoeken of de grootte van het weefselmonster de resultaten van homogenisatie beïnvloedt.

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Biologie Nummer 173 nitraat nitriet stikstofmonoxide skeletspieren weefselhomogenisatie
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