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Journal / Biochemistry / Shotgun Proteomics Probenverarbeitung…
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Biochemistry

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JoVE Journal Biochemistry
Shotgun Proteomics Probenverarbeitung automatisiert durch einen Open-Source-Laborroboter
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
Journal (Biochemistry)
Laser-free Hydroxyl Radical Protein Footprinting to Perform Higher Order Structural Analysis of Proteins
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
Journal (Biochemistry)
Characterization of Glycoproteins with the Immunoglobulin Fold by X-Ray Crystallography and Biophysical Techniques
 
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Shotgun Proteomics Probenverarbeitung automatisiert durch einen Open-Source-Laborroboter

Article DOI: 10.3791/63092-v • 10:12 min • October 28th, 2021
October 28th, 2021 •
1,3, 1,3, 1,3, 1,3, 1,2,3
1Department of Medicine-Cardiology, University of Colorado Anschutz Medical Campus, 2Department of Biochemistry & Molecular Genetics, University of Colorado Anschutz Medical Campus, 3Consortium for Fibrosis Research & Translation, School of Medicine, University of Colorado Anschutz Medical Campus

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Detailliertes Protokoll und drei Python-Skripte werden für den Betrieb eines Open-Source-Roboter-Liquid-Handling-Systems bereitgestellt, um eine halbautomatische Proteinprobenvorbereitung für Massenspektrometrie-Experimente durchzuführen, die die Entfernung von Reinigungsmitteln, die Proteinverdauung und die Peptidentsalzungsschritte abdecken.

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Biochemie Heft 176
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