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Ensaios de ACT1-CUP1 determinam as sensibilidades específicas do substrato de mutantes spliceossomais em leveduras em brotamento
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Ensaios de ACT1-CUP1 determinam as sensibilidades específicas do substrato de mutantes spliceossomais em leveduras em brotamento

Article DOI: 10.3791/63232-v • 07:31 min • June 30th, 2022
June 30th, 2022 •
1
1College of Arts and Sciences, Northwest University

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O ensaio ACT1-CUP1, um ensaio de crescimento de cobre, fornece uma leitura rápida do splicing de RNA mensageiro precursor (pré-mRNA) e do impacto que os fatores de splicing mutantes têm na função spliceossomal. Este estudo fornece um protocolo e destaca a personalização possível para abordar a questão do splicing de interesse.

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Genética Edição 184 splicing pré-RNAm Saccharomyces cerevisiae spliceossoma ACT1-CUP1 ensaio de crescimento tela genética tela mutante sequência não consensual
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