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成虫 のアノフェレスガンビアエ 蚊への効果的な経口RNA干渉(RNAi)投与
A Mouse 5/6th Nephrectomy Model That Induces Experimental Uremic Cardiomyopathy
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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Journal (Biology)
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成虫 のアノフェレスガンビアエ 蚊への効果的な経口RNA干渉(RNAi)投与

Article DOI: 10.3791/63266-v • 07:48 min • March 1st, 2022
March 1st, 2022 •
1,2, 1, 3, 4,5, 5,6, 1, 5,7,8
1Division of Parasitic Diseases and Malaria, Entomology Branch, Centers for Disease Control and Prevention, 2Department of Entomology, Cornell University, 3Centro de Estudios en Biotecnologia, Universidad del Valle de Guatemala, 4Department of Cell Biology, Johns Hopkins School of Medicine, 5Johns Hopkins Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health, 6Department of Molecular Microbiology and Immunology, Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute, 7Department of Cell Biology, Johns Hopkins School of Medicine, 8Biomedical Sciences Department, Idaho College of Osteopathic Medicine

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細菌によって産生されるdsRNAの経口投与は、 カエノラブディティス・エレガンス において日常的に使用されているRNA干渉(RNAi)の送達方法であり、ここで成虫の蚊に適用することに成功した。我々の方法は、注射を使用せずに堅牢な逆遺伝学研究および伝達遮断ベクター研究を可能にする。

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生物学 第181号
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