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JoVE Journal Medicine
Modificeret teknik til brug af neonatale murinhjerter i Langendorff-præparatet
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Modificeret teknik til brug af neonatale murinhjerter i Langendorff-præparatet

Article DOI: 10.3791/63349-v • 08:23 min • March 4th, 2022
March 4th, 2022 •
1, 2
1Department of Anesthesiology, Vanderbilt University Medical Center, 2Department of Anesthesiology, Columbia University Medical Center

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Den nuværende protokol beskriver aorta cannulation og retrograd perfusion af ex-vivo neonatal murine hjerte. En to-personers strategi ved hjælp af et dissekeringsmikroskop og en stump lille målernål tillader pålidelig kannulation. Kvantificering af langsgående kontraktilspænding opnås ved anvendelse af en krafttransducer forbundet med toppen af venstre ventrikel.

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Medicin udgave 181 Aorta kannulation ex-vivo kraft hjerte iskæmi-reperfusion isoleret Langendorff mus nyfødt retrograd perfusion ventrikulær
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