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JoVE Journal Neuroscience
Im lebenden Organismus Weitfeld- und Zwei-Photonen-Kalzium-Bildgebung einer Maus mit einem großen Schädelfenster
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Im lebenden Organismus Weitfeld- und Zwei-Photonen-Kalzium-Bildgebung einer Maus mit einem großen Schädelfenster

Article DOI: 10.3791/64224-v • 06:45 min • August 4th, 2022
August 4th, 2022 •
1, 2,3, 4, 2,3, 1
1Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 2Department of Neuropharmacology, Faculty of Medicine, University of Yamanashi, 3Yamanashi GLIA center, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 4Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo

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Das vorliegende Protokoll beschreibt die Herstellung eines großen (6 x 3 mm2) Schädelfensters mit Lebensmittelverpackung, transparentem Silikon und Deckglas. Dieses Schädelfenster ermöglicht in vivo Weitfeld- und Zwei-Photonen-Kalzium-Bildgebungsexperimente in derselben Maus.

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Neuroscience Ausgabe 186
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