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Biochemistry

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JoVE Journal Biochemistry
En "dual-addition" kalciumfluorescensanalys för screening med hög genomströmning av rekombinanta G-proteinkopplade receptorer
Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses
Journal (Biochemistry)
Author Spotlight: Unlocking the World of Intrinsically Disordered Regions with Cellular Sensing and Responses
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
Journal (Biochemistry)
Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
 
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En "dual-addition" kalciumfluorescensanalys för screening med hög genomströmning av rekombinanta G-proteinkopplade receptorer

Article DOI: 10.3791/64505-v • 08:46 min • December 2nd, 2022
December 2nd, 2022 •
1, 2, 1
1Department of Entomology, Texas A&M University, 2Department of Biochemistry and Biophysics, Texas A&M University

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I detta arbete beskrivs en intracellulär kalciumfluorescensanalys med hög genomströmning för 384-brunnsplattor för att screena små molekylbibliotek på rekombinanta G-proteinkopplade receptorer (GPCR). Målet, kininreceptorn från nötkreatursfeberfästingen, Rhipicephalus microplus, uttrycks i CHO-K1-celler. Denna analys identifierar agonister och antagonister som använder samma celler i en "dual-addition" -analys.

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Biokemi utgåva 190
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