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JoVE Journal Cancer Research
Modellering af oral-esophageal pladecellekarcinom i 3D-organoider
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Modellering af oral-esophageal pladecellekarcinom i 3D-organoider

Article DOI: 10.3791/64676-v • 10:43 min • December 23rd, 2022
December 23rd, 2022 •
*1, *1,2, 1, 1, 1, 1, 1, 1,3, 1,3, 4, 1,2, 1,5, 1,6, 1,7, 1,5, 1,2,5
1Herbert Irving Comprehensive Cancer Center, Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, 3Department of Otolaryngology, Head and Neck Surgery, Columbia University, 4Histopathology Facility, Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, 6Department of Genetics and Development, Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University
* These authors contributed equally

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Denne protokol beskriver de vigtigste trin til at generere og karakterisere murin oral-esophageal 3D-organoider, der repræsenterer normale, præneoplastiske og pladecellekarcinomlæsioner induceret via kemisk carcinogenese.

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Kræftforskning nr. 190
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