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JoVE Journal Chemistry
In vitro og in vivo evaluering av fotokontrollerte biologisk aktive forbindelser - potensielle legemiddelkandidater for fotofarmakologi av kreft
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In vitro og in vivo evaluering av fotokontrollerte biologisk aktive forbindelser - potensielle legemiddelkandidater for fotofarmakologi av kreft

Article DOI: 10.3791/64902-v • 13:17 min • September 29th, 2023
September 29th, 2023 •
*1,2, *2, 2, 1, 1, 1, 2, 2, 3,4, 3, 4, 3, 1,2,4
1Taras Shevchenko National University of Kyiv, 2Bienta/Enamine, 3Karlsruhe Institute of Technology, 4Lumobiotics
* These authors contributed equally

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Denne protokollen presenterer et sett med eksperimenter vedtatt for evaluering av fotoswitchable anticancer peptider, som kan brukes i preklinisk screening av slike forbindelser. Dette inkluderer cytotoksisitetsvurdering i 2D- og 3D-cellekulturer, evaluering av fotoisomeriseringseffektivitet ex vivo (modellvev) og in vivo-effekt.

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