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人瘢痕疙瘩组织中原代真皮成纤维细胞的分离、培养和表征
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Medicine
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JoVE Journal
Medicine
Isolation, Culture, and Characterization of Primary Dermal Fibroblasts from Human Keloid Tissue
Please note that all translations are automatically generated.
Click here for the English version.
人瘢痕疙瘩组织中原代真皮成纤维细胞的分离、培养和表征
DOI:
10.3791/65153-v
•
04:41 min
•
July 28, 2023
•
Jinru Song
,
Yue Zhang
,
Huihui Pan
,
Xueyan Xu
,
Cheng-Cheng Deng
,
Bin Yang
1
Dermatology Hospital
,
Southern Medical University
Chapters
00:04
Introduction
00:35
Tissue Preparation and Isolation
01:33
Tissue Culture and Identification of Fibroblasts by Immunofluorescence Staining
03:29
Results: Characterized Fibroblastic Growth
04:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本研究描述了一种优化的方案,用于从瘢痕疙瘩组织中建立原代成纤维细胞,可以有效和稳定地提供纯净和可行的成纤维细胞。
Tags
Keloid
Fibroblasts
Primary Cell Culture
Immunofluorescence
DAPI
Centrifugation
Trypsin-EDTA
DMEM
FBS
PSA
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