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JoVE Journal Bioengineering
DNA-tjudrat RNA-polymeras för programmerbar in vitro-transkription och molekylär beräkning
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DNA-tjudrat RNA-polymeras för programmerbar in vitro-transkription och molekylär beräkning

Article DOI: 10.3791/62073-v • 09:26 min • December 29th, 2021
December 29th, 2021 •
1, 1, 1
1Institute of Biomedical Engineering, University of Toronto

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Vi beskriver tekniken av en ny DNA-tjudrade T7 RNA polymeras för att reglera in vitro transkription reaktioner. Vi diskuterar stegen för proteinsyntes och karakterisering, validerar proof-of-concept transkriptionell reglering och diskuterar dess tillämpningar inom molekylär databehandling, diagnostik och molekylär informationsbehandling.

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Bioengineering nummer 178 dynamisk DNA-nanoteknik molekylär programmering molekylär databehandling in vitro-genkrets in vitro-transkription tåmedierad strandförskjutning
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