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JoVE Journal Biology
Un método paso a paso para detectar anticuerpos neutralizantes contra AAV utilizando un ensayo colorimétrico basado en células
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Un método paso a paso para detectar anticuerpos neutralizantes contra AAV utilizando un ensayo colorimétrico basado en células

Article DOI: 10.3791/63419-v • 05:53 min • December 7th, 2021
December 7th, 2021 •
1,2, 2,3, 3, 4, 1,5,6, 1,2,5,6,7
1Baker Heart and Diabetes Institute, 2Department of Physiology, Anatomy and Microbiology, La Trobe University, 3Florey Institute of Neuroscience and Mental Health, University of Melbourne, 4Department of Physiology, Centre for Muscle Research (CMR), The University of Melbourne, 5Department of Diabetes, Central Clinical School, Monash University, 6Baker Department of Cardiometabolic Health, The University of Melbourne, 7Department of Physiology and Department of Medicine Alfred Hospital, Monash University

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Se describe un protocolo de laboratorio completo y un flujo de trabajo de análisis para un ensayo basado en células colorimétricas rápidas, rentables y sencillas para detectar elementos neutralizantes contra AAV6.

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Biología Número 178
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