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Journal / Biology / Single-Nucleus RNA 시퀀싱을 위한 성인 마우스 신장에서 핵…
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Biology

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Single-Nucleus RNA 시퀀싱을 위한 성인 마우스 신장에서 핵 분리

Article DOI: 10.3791/62901-v • 06:00 min • September 20th, 2021
September 20th, 2021 •
*1,2, *1,2, 3, 4, 3, 3, 1,2
1Department of Nephrology and Medical Intensive Care Medicine, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, 2Molecular and Translational Kidney Research, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 3Berlin Institute of Medical Systems Biology (BIMSB), Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), 4Berlin Institute of Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC)
* These authors contributed equally

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여기에서 우리는 수질 신장 세포 유형의 표현을 개선하고 효소 조직 해리로 인한 유전자 발현 아티팩트를 방지하는 냉동 마우스 신장에서 고품질 핵을 분리하는 프로토콜을 제시합니다.

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생물학 제 175 호 신장 핵 분리 전사체학 전사체 시퀀싱 단일 핵 RNA 시퀀싱 snRNA-seq
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