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Using Induced Pluripotent Stem Cell Suspension for Kidney Organoid Production

September 1st, 2023
*1,2, *3, 4, 2, 1, 1, 1, 1, 5, 1, 1,2
1Department of Nephrology, The Children's Hospital, Zhejiang University School of Medicine, 2Zhejiang University School of Medicine, 3Hubei Normal University, 4National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University School of Medicine, 5Clinical Laboratory, The Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health
* These authors contributed equally

Summary
Automatic Translation

Denne protokol præsenterer en omfattende og effektiv metode til fremstilling af nyreorganoider fra inducerede pluripotente stamceller (iPSC'er) under anvendelse af suspensionskulturbetingelser. Den primære vægt i denne undersøgelse ligger i bestemmelsen af den indledende celletæthed og WNT-agonistkoncentrationen, hvilket gavner forskere, der er interesseret i nyreorganoidforskning.

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