Materials
Name | Company | Catalog Number | Comments |
Millicell Cell Culture Insert, 12 mm, hydrophilic PTFE (Biopore), 0.4 μm |
Millipore | PICM01250 | |
Insulin syringe, 1 ml | Beckton Dickinson | 309659 | |
Scissors | Fine Science Tools | 15003-08 | dissection |
Forceps, Dumont #55, inox | Fine Science Tools | 11255-20 | dissection |
Cryostat | Leica | various | |
Confocal Microscope System | Nikon | various | |
Capillary glass | World Precision Instruments | 1B150F-4 | |
P-97 Flaming/Brown Micropipette Puller | Sutter Instrument Co. | ||
Picospritzer III | Parker Hannifin | ||
Glass Bottom Culture Dishes | MatTek Corporation | P35G-0-14-C | |
Petri dish | Falcon | 1008 | |
Disposable Graduated Transfer Pipettes | VWR | 16001-180 | |
Multiwell plates, 24-well | Beckton Dickinson | 351147 | |
Cover glass, #1 | Electron Microscopy Sciences | 72200-30 | |
Polysine adhesion slides | Electron Microscopy Sciences | 63412-01 | |
Microscope slides | Globe | 1301 | |
Liquid Blocker | Electron Microscopy Sciences | 71312 | |
VECTASHIELD mounting medium | Vector Laboratories | H-1000 | |
OCT medium | Sakura | 4583 | |
Parafilm laboratory film | Fisher | 13-374-10 | |
Dow Corning high vacuum grease | Sigma-Aldrich | Z273554 | |
Mouse anti-PSD95 | Millipore | MABN68 | antibody |
Donkey anti-mouse Alexa 568 | Invitrogen | A10037 | antibody |
Isolectin Alexa 488 | Invitrogen | I21411 | |
Chemiblocker | Chemicon | 2170S | |
Triton X-100 | Sigma-Aldrich | T9284 | |
Sulforhodamine | Sigma-Aldrich | 341738 | |
Carbodiimide | Thermo Scientific | 22980 | EDC |
Paraformaldehyde | Sigma-Aldrich | P6148 |
References
- Csaba, G., Kovacs, P., Pallinger, E. EDAC fixation increases the demonstrability of biogenic amines in the unicellular Tetrahymena: a flow cytometric and confocal microscopic comparative analysis. Cell Biol. Int. 30, 345-348 (2006).
- Fletcher, E. L., Hack, I., Brandstatter, J. H., Wassle, H. Synaptic localization of NMDA receptor subunits in the rat retina. J. Comp. Neurol. 420, 98-112 (2000).
- Gastinger, M. J., O'Brien, J. J., Larsen, N. B., Marshak, D. W. Histamine immunoreactive axons in the macaque retina. Invest. Ophthalmol. Vis. Sci. 40, 487-495 (1999).
- Haverkamp, S., Wassle, H.
Immunocytochemical analysis of the mouse retina. J. Comp. Neurol. 424, 1-23 (2000). - Koulen, P., Fletcher, E. L., Craven, S. E., Bredt, D. S., Wassle, H. Immunocytochemical localization of the postsynaptic density protein PSD-95 in the mammalian retina. J. Neurosci. 18, 10136-10149 (1998).
- Pena, J. T., et al. miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nat. Methods. 6, 139-141 (2009).
- Tymianski, M., et al. A novel use for a carbodiimide compound for the fixation of fluorescent and non-fluorescent calcium indicators in situ following physiological experiments. Cell Calcium. 21, 175-183 (1997).
- Sagdullaev, B. T., Eggers, E. D., Purgert, R., Lukasiewicz, P. D. Nonlinear interactions between excitatory and inhibitory retinal synapses control visual output. J. Neurosci. 31, 15102-15112 (2011).
- Toychiev, A. H., Sagdullaev, B., Yee, C. W., Ivanova, E., Sagdullaev, B. T. A time and cost efficient approach to functional and structural assessment of living neuronal tissue. J. Neurosci. Methods. 214, 105-112 (2013).
- Yee, C. W., Toychiev, A. H., Sagdullaev, B. T. Network deficiency exacerbates impairment in a mouse model of retinal degeneration. Front Syst. Neurosci. 6, 8 (2012).
- Ivanova, E., Muller, U., Wassle, H. Characterization of the glycinergic input to bipolar cells of the mouse retina. Eur. J. Neurosci. 23, 350-364 (2006).
- Dmitrieva, N. A., Strang, C. E., Keyser, K. T. Expression of alpha 7 nicotinic acetylcholine receptors by bipolar, amacrine, and ganglion cells of the rabbit retina. J. Histochem. Cytochem. 55, 461-476 (2007).