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Journal / Neuroscience / El análisis del transcriptoma de células…
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Neuroscience

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JoVE Journal Neuroscience
El análisis del transcriptoma de células individuales
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El análisis del transcriptoma de células individuales

Article DOI: 10.3791/2634-v • 07:27 min • April 25th, 2011
April 25th, 2011 •
*1, *1, 1,2
1Department of Pharmacology, University of Pennsylvania, 2The Penn Genome Frontiers Institute, University of Pennsylvania
* These authors contributed equally

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En este artículo se describe un método simple para la recolección de las células individuales de rata cultivos primarios de neuronas y posterior análisis de transcriptoma utilizando la amplificación ARNA. Este enfoque es generalizable a cualquier tipo de célula.

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Neurociencia Número 50 de una sola célula transcriptoma Arna amplificación RT-PCR biología molecular la expresión de genes
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