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Journal / Genetics / 利用下一代序列染色体构象捕获 (4 c 序列)…
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Genetics

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JoVE Journal Genetics
利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列
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Journal (Genetics)
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
Journal (Genetics)
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
 
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利用下一代序列染色体构象捕获 (4 c 序列) 的高通量识别基因调控数列

Article DOI: 10.3791/58030-v • 09:06 min • October 5th, 2018
October 5th, 2018 •
1, 1, 1
1Division of Dermatology, Center for Pharmacogenomics, Center for the Study of Itch, Department of Medicine, Washington University School of Medicine

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基因和调控元素之间的物理相互作用的识别是具有挑战性的, 但已被染色体构象捕获方法所促进。这种对 4 c 序列协议的修改可以减少 pcr 模板的过度放大, 并通过加入限制酶消化步骤来最大限度地提高 mappability 的读数。

Tags

遗传学 问题 140 染色质 循环 增强剂 调节 排序 诱饵 限制酶 裂解
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