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JoVE Journal Biochemistry
DNA-sequentie herkenning door DNA primase met behulp van high-throughput Primase profilering
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DNA-sequentie herkenning door DNA primase met behulp van high-throughput Primase profilering

Article DOI: 10.3791/59737-v • 08:04 min • October 8th, 2019
October 8th, 2019 •
1, 1, 2, 2, 1, 1
1Department of Chemistry, Ben-Gurion University of the Negev, 2Center for Genomic and Computational Biology, Department of Biostatistics and Bioinformatics, Duke University

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Experimenten met eiwitbinding Microarray (PBM) in combinatie met biochemische assays koppelen de bindende en katalytische eigenschappen van DNA primase, een enzym dat RNA-primers op Template DNA synthetiseert. Deze methode, aangeduid als high-throughput Primase profilering (HTPP), kan worden gebruikt om DNA-bindende patronen van een verscheidenheid aan enzymen te onthullen.

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Biochemie uitgave 152 hoge doorvoer Primase profilering HTPP proteïne binding Microarray PBM DNA primase DNA-replicatie eiwit-DNA-interactie DNA-binding RNA primers moleculaire biologie
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