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Een Ectopic Chemokine-expressie model voor het testen van macrophage Recruitment in vivo

Article DOI: 10.3791/60161-v • 10:02 min • September 25th, 2019
September 25th, 2019 •
1, 1, 2
1Department of Developmental Biology, School of Basic Medical Sciences, Southern Medical University, 2Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology

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Om het effect van een Chemokine op macrofaag werving in vivo te testen, werd de hele mount in situ-hybridisatie gebruikt om de ectopische uitdrukking van de Chemokine te detecteren en werd immunokleuring gebruikt om macrofagen te labelen. Live Imaging werd gebruikt voor real-time observatie van macrofaag migratie.

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Ontwikkelingsbiologie uitgave 151 hele Mount fluorescentie in situ hybridisatie immunokleuring Live Imaging zebravis IL-34 macrofaag
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