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Monitorando o conjunto eIF4F medindo a interação eIF4E-eIF4G em células vivas
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Monitorando o conjunto eIF4F medindo a interação eIF4E-eIF4G em células vivas

Article DOI: 10.3791/60850-v • 08:47 min • May 1st, 2020
May 1st, 2020 •
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1p53 Laboratory, Neuros/Immunos, A*STAR (Agency for Science, Technology and Research)

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Aqui, apresentamos um protocolo para medir a interação eIF4E-eIF4G em células vivas que permitiria ao usuário avaliar a perturbação induzida por drogas da dinâmica complexa eIF4F em formatos de triagem.

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Pesquisa sobre câncer Questão 159 Biologia Celular complexo eIF4F controle translacional ensaio de complementação da luciferase sinalização mTOR interações proteína-proteína
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