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Biochemistry

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JoVE Journal Biochemistry
Complementação da Atividade de Emenda por um Complexo Galectin-3 - U1 snRNP on Beads
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Complementação da Atividade de Emenda por um Complexo Galectin-3 - U1 snRNP on Beads

Article DOI: 10.3791/61990-v • 08:48 min • December 9th, 2020
December 9th, 2020 •
1, 1, 2, 1
1Department of Biochemistry and Molecular Biology, Michigan State University, 2Department of Microbiology and Molecular Genetics, Michigan State University

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Este artigo descreve os procedimentos experimentais para (a) esgotamento do U1 snRNP a partir de extratos nucleares, com perda concomitante de atividade de emenda; e (b) reconstituição da atividade de emenda no extrato empobrecido U1 por partículas de galectina-3 - U1 snRNP ligadas a contas covalentemente acopladas a anticorpos anti-galectina-3.

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Bioquímica Edição 166 emenda pré-mRNA emenda ensaio de emenda livre de células fracionamento de gradiente de glicerol complexo U1 snRNP galectinas
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