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Journal / Biochemistry / Myosine-specifieke aanpassingen van in vitro…
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Biochemistry

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JoVE Journal Biochemistry
Myosine-specifieke aanpassingen van in vitro fluorescentiemicroscopie-gebaseerde motiliteitstesten
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Myosine-specifieke aanpassingen van in vitro fluorescentiemicroscopie-gebaseerde motiliteitstesten

Article DOI: 10.3791/62180-v • 08:57 min • February 4th, 2021
February 4th, 2021 •
1, 1,2, 1, 1, 1
1Laboratory of Molecular Physiology, Cell and Developmental Biology Center, National Heart, Lung and Blood Institute, National Institutes of Health, 2Department of Physiology, Perelman School of Medicine, University of Pennsylvania

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Hier wordt een procedure gepresenteerd om myosine 5a uit te drukken en te zuiveren, gevolgd door een bespreking van de karakterisering ervan, met behulp van zowel ensemble- als single molecule in vitro fluorescentiemicroscopie-gebaseerde assays, en hoe deze methoden kunnen worden aangepast voor de karakterisering van niet-amuskel myosine 2b.

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Biochemie myosine actine filamenten eiwitzuivering fluorescentie microscopie motiliteitstest single molecule assay bipolaire filamenten totale interne reflectie fluorescentie microscopie
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