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Journal / Biochemistry / Studi di interazioni Chaperone-Cochaperone…
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Biochemistry

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JoVE Journal Biochemistry
Studi di interazioni Chaperone-Cochaperone utilizzando il saggio omogeneo basato su perle
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Studi di interazioni Chaperone-Cochaperone utilizzando il saggio omogeneo basato su perle

Article DOI: 10.3791/62762-v • 06:51 min • July 21st, 2021
July 21st, 2021 •
1, 1, 1,2, 1,3, 1
1Department of Neuroscience Care and Society, Division of Neurogeriatrics, Karolinska Institutet, 2Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (BHU), 3Theme Inflammation and Aging, Karolinska University Hospital

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Questo protocollo presenta una tecnica per sondare le interazioni proteina-proteina utilizzando perle donatrici legate al glutatione con co-chaperoni tPR fusi GST e perle accettori accoppiate con un peptide derivato da Hsp90. Abbiamo usato questa tecnica per lo screening di piccole molecole per interrompere le interazioni Hsp90-FKBP51 o Hsp90-FKBP52 e identificato inibitori di interazione Hsp90-FKBP51 potenti e selettivi.

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Biochimica AlphaScreen interazioni proteina-proteina chaperone molecolare Hsp90 FKBP51 FKBP52 proteine TPR screening composto
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