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JoVE Journal Cancer Research
Microscopia a fluorescenza per l'internalizzazione dell'ATP mediata dalla macropinocitosi in cellule tumorali umane e topi xenotrapiantati da tumore
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Microscopia a fluorescenza per l'internalizzazione dell'ATP mediata dalla macropinocitosi in cellule tumorali umane e topi xenotrapiantati da tumore

Article DOI: 10.3791/62768-v • 08:31 min • June 30th, 2021
June 30th, 2021 •
*1,2,3, *4, 1,5, 4, 1,2,4, 1,2,4, 6, 1,2,4,7
1Department of Biological Sciences, Ohio University, 2Molecular and Cellular Biology Program, Ohio University, 3Neuroscience Program, Ohio University, 4Edison Biotechnology Institute, Ohio University, 5Translational Biomedical Sciences Program, Ohio University, 6Honors Tutorial College, Ohio University, 7Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University
* These authors contributed equally

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Abbiamo sviluppato un metodo riproducibile per visualizzare l'internalizzazione dell'adenosina trifosfato fluorescente non idrolizzabile (ATP), un surrogato di ATP, ad alta risoluzione cellulare. Abbiamo convalidato il nostro metodo utilizzando saggi indipendenti in vitro e in vivo: linee cellulari tumorali umane e topi immunodeficienti xenografati con tessuto tumorale umano.

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