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JoVE Journal Biochemistry
Beoordeling van submitchondrial protein localization in ontluikende gist Saccharomyces cerevisiae
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Beoordeling van submitchondrial protein localization in ontluikende gist Saccharomyces cerevisiae

Article DOI: 10.3791/62853-v • 08:55 min • July 19th, 2021
July 19th, 2021 •
1, 1, 1, 1
1Departamento de Genética e Biologia Evolutiva, Instituto de Biociências, Universidade de São Paulo

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Ondanks recente vooruitgang blijven veel mitochondriale gisteiwitten nog steeds met hun functies volledig onbekend. Dit protocol biedt een eenvoudige en betrouwbare methode om de submitchondriale lokalisatie van eiwitten te bepalen, wat fundamenteel is geweest voor de opheldering van hun moleculaire functies.

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Biochemie Nummer 173 mitochondriën ontluikende gist Saccharomyces cerevisiae submitochondrial compartimenten submitchondrial fractionering submitochondrial lokalisatie sonicatie carbonaat extractie western blot
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