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Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System
DOI:

09:39 min

April 15, 2012

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Chapters

  • 00:02Title
  • 00:48Electrophysiology rig set-up
  • 01:18Nanoinjection set-up
  • 03:14Drosophila melanogaster preparation
  • 03:58Paired nanoinjection/electrophysiology
  • 07:35Results

Summary

Automatic Translation

A rapid in vivo assay to test for neuromodulatory compounds using the Giant Fiber System (GFS) of Drosophila melanogaster is described. Nanoinjections in the head of the animal along with electrophysiological recordings of the GFS can reveal bioactivity of compounds on neurons or muscles.

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