Achillefs N. Kapanidis Clarendon Laboratory, Department of Physics University of Oxford Biography Publications Institution JoVE Articles Achillefs N. Kapanidis has not added a biography. If you are Achillefs N. Kapanidis and would like to personalize this page please email our Author Liaison for assistance. Publications Tethered Fluorophore Motion: Studying Large DNA Conformational Changes by Single-fluorophore Imaging Biophysical Journal. Sep, 2014 | Pubmed ID: 25185556 Single-molecule FRET Reveals a Corkscrew RNA Structure for the Polymerase-bound Influenza Virus Promoter Proceedings of the National Academy of Sciences of the United States of America. Aug, 2014 | Pubmed ID: 25071209 In Vivo Single-molecule Imaging of Bacterial DNA Replication, Transcription, and Repair FEBS Letters. Oct, 2014 | Pubmed ID: 24859634 Studying the Organization of DNA Repair by Single-cell and Single-molecule Imaging DNA Repair. Aug, 2014 | Pubmed ID: 24629485 Long-lived Intracellular Single-molecule Fluorescence Using Electroporated Molecules Biophysical Journal. Dec, 2013 | Pubmed ID: 24314075 Conformational Transitions During FtsK Translocase Activation of Individual XerCD-dif Recombination Complexes Proceedings of the National Academy of Sciences of the United States of America. Oct, 2013 | Pubmed ID: 24101525 Conformational Landscapes of DNA Polymerase I and Mutator Derivatives Establish Fidelity Checkpoints for Nucleotide Insertion Nature Communications. 2013 | Pubmed ID: 23831915 Multiscale Spatial Organization of RNA Polymerase in Escherichia Coli Biophysical Journal. Jul, 2013 | Pubmed ID: 23823236 Rotavirus MRNAS Are Released by Transcript-specific Channels in the Double-layered Viral Capsid Proceedings of the National Academy of Sciences of the United States of America. Jul, 2013 | Pubmed ID: 23818620 Single-molecule DNA Repair in Live Bacteria Proceedings of the National Academy of Sciences of the United States of America. May, 2013 | Pubmed ID: 23630273 Non-covalent Single Transcription Factor Encapsulation Inside a DNA Cage Angewandte Chemie (International Ed. in English). Feb, 2013 | Pubmed ID: 23325751 The Transcription Bubble of the RNA Polymerase-promoter Open Complex Exhibits Conformational Heterogeneity and Millisecond-scale Dynamics: Implications for Transcription Start-site Selection Journal of Molecular Biology. Mar, 2013 | Pubmed ID: 23274143 Six Steps Closer to FRET-driven Structural Biology Nature Methods. Dec, 2012 | Pubmed ID: 23223168 Capturing Reaction Paths and Intermediates in Cre-loxP Recombination Using Single-molecule Fluorescence Proceedings of the National Academy of Sciences of the United States of America. Dec, 2012 | Pubmed ID: 23184986 T7 RNA Polymerase Functions in Vitro Without Clustering PloS One. 2012 | Pubmed ID: 22768341 Characterization of Dark Quencher Chromophores As Nonfluorescent Acceptors for Single-molecule FRET Biophysical Journal. Jun, 2012 | Pubmed ID: 22713582 A Protein Biosensor That Relies on Bending of Single DNA Molecules Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Mar, 2012 | Pubmed ID: 22368093 Colorful Molecular Diagnostics Clinical Chemistry. Apr, 2012 | Pubmed ID: 22300628 DAOSTORM: an Algorithm for High- Density Super-resolution Microscopy Nature Methods. Apr, 2011 | Pubmed ID: 21451515 Identifying Molecular Dynamics in Single-molecule FRET Experiments with Burst Variance Analysis Biophysical Journal. Mar, 2011 | Pubmed ID: 21402040 Improved Temporal Resolution and Linked Hidden Markov Modeling for Switchable Single-molecule FRET Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Feb, 2011 | Pubmed ID: 21280168 Defining the Limits of Single-molecule FRET Resolution in TIRF Microscopy Biophysical Journal. Nov, 2010 | Pubmed ID: 21044609 Sensing DNA Opening in Transcription Using Quenchable Förster Resonance Energy Transfer Biochemistry. Nov, 2010 | Pubmed ID: 20818825 Monitoring Multiple Distances Within a Single Molecule Using Switchable FRET Nature Methods. Oct, 2010 | Pubmed ID: 20818380 Surfing on a New Wave of Single-molecule Fluorescence Methods Physical Biology. 2010 | Pubmed ID: 20686191 Characterizing Single-molecule FRET Dynamics with Probability Distribution Analysis Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Jul, 2010 | Pubmed ID: 20575136 Conformational Transitions in DNA Polymerase I Revealed by Single-molecule FRET Proceedings of the National Academy of Sciences of the United States of America. Jan, 2010 | Pubmed ID: 20080740 Single-molecule DNA Biosensors for Protein and Ligand Detection Angewandte Chemie (International Ed. in English). Feb, 2010 | Pubmed ID: 20077444 Probing Biomolecular Structures and Dynamics of Single Molecules Using In-gel Alternating-laser Excitation Analytical Chemistry. Dec, 2009 | Pubmed ID: 19863108 DNA Monofunctionalization of Quantum Dots Chembiochem : a European Journal of Chemical Biology. Jul, 2009 | Pubmed ID: 19554595 A Facile Method for Reversibly Linking a Recombinant Protein to DNA Chembiochem : a European Journal of Chemical Biology. Jun, 2009 | Pubmed ID: 19449345 Single-molecule FRET Analysis of Protein-DNA Complexes Methods in Molecular Biology (Clifton, N.J.). 2009 | Pubmed ID: 19378183 Biology, One Molecule at a Time Trends in Biochemical Sciences. May, 2009 | Pubmed ID: 19362843 Reconfigurable, Braced, Three-dimensional DNA Nanostructures Nature Nanotechnology. Feb, 2008 | Pubmed ID: 18654468 Red Light, Green Light: Probing Single Molecules Using Alternating-laser Excitation Biochemical Society Transactions. Aug, 2008 | Pubmed ID: 18631150 Periodic Acceptor Excitation Spectroscopy of Single Molecules European Biophysics Journal : EBJ. Jul, 2007 | Pubmed ID: 17279362 Three-color Alternating-laser Excitation of Single Molecules: Monitoring Multiple Interactions and Distances Biophysical Journal. Jan, 2007 | Pubmed ID: 17040983 Initial Transcription by RNA Polymerase Proceeds Through a DNA-scrunching Mechanism Science (New York, N.Y.). Nov, 2006 | Pubmed ID: 17110578 Direct Observation of Abortive Initiation and Promoter Escape Within Single Immobilized Transcription Complexes Biophysical Journal. Feb, 2006 | Pubmed ID: 16299085 Retention of Transcription Initiation Factor Sigma70 in Transcription Elongation: Single-molecule Analysis Molecular Cell. Nov, 2005 | Pubmed ID: 16285917 Alternating-laser Excitation of Single Molecules Accounts of Chemical Research. Jul, 2005 | Pubmed ID: 16028886 Accurate FRET Measurements Within Single Diffusing Biomolecules Using Alternating-laser Excitation Biophysical Journal. Apr, 2005 | Pubmed ID: 15653725 Fluorescence-aided Molecule Sorting: Analysis of Structure and Interactions by Alternating-laser Excitation of Single Molecules Proceedings of the National Academy of Sciences of the United States of America. Jun, 2004 | Pubmed ID: 15175430 Fluorescence Resonance Energy Transfer (FRET) in Analysis of Transcription-complex Structure and Function Methods in Enzymology. 2003 | Pubmed ID: 14712697 The Power and Prospects of Fluorescence Microscopies and Spectroscopies Annual Review of Biophysics and Biomolecular Structure. 2003 | Pubmed ID: 12598370 Structural Organization of Bacterial RNA Polymerase Holoenzyme and the RNA Polymerase-promoter Open Complex Cell. Mar, 2002 | Pubmed ID: 11893332 Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation Louise Aigrain1,2, Marko Sustarsic1, Robert Crawford1, Anne Plochowietz1, Achillefs N. Kapanidis1 1Clarendon Laboratory, Department of Physics, University of Oxford, 2Wellcome Trust Sanger Institute, Genome Center JoVE 52208 Biology Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking Stephan Uphoff1,2, David J. Sherratt1, Achillefs N. Kapanidis2 1Microbiology Unit, Department of Biochemistry, University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford JoVE 51177 Immunology and Infection
Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation Louise Aigrain1,2, Marko Sustarsic1, Robert Crawford1, Anne Plochowietz1, Achillefs N. Kapanidis1 1Clarendon Laboratory, Department of Physics, University of Oxford, 2Wellcome Trust Sanger Institute, Genome Center JoVE 52208 Biology
Visualizing Protein-DNA Interactions in Live Bacterial Cells Using Photoactivated Single-molecule Tracking Stephan Uphoff1,2, David J. Sherratt1, Achillefs N. Kapanidis2 1Microbiology Unit, Department of Biochemistry, University of Oxford, 2Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford JoVE 51177 Immunology and Infection