Juan Carlos Zapata School of Medicine-University of Maryland. University of Maryland Biography Publications Institution JoVE Articles Juan Carlos ZapataResearch Associate My main research interest is in virus-host interactions using animal models, genomic profiling, and basic molecular virology. I focused not only on host responses but also on changes that occur in the viral population in order to describe virus-host coevolution. I identified mutations in the arenavirus nucleocapsid protein (NP) that are likely to impact viral pathogenesis and the induction of protective immunity. During my postdoctoral training, I worked in detecting and characterizing HIV viral factors related with latency in the reservoir cells and in implanting patient-derived ATL cells into NSG mice to study cancer pathogenesis and treatment options. Currently, I am using humanized animal models to study infectious diseases (HIV, HTLV, HBV, HCV, and Dengue), human cancers and graft vs host disease (GvHD). I have also detected and characterized a CD8+ T cell sub-population, in humanized mice, that correlated with HIV protection in human beings. Publications A Single Dose of Modified Vaccinia Ankara Expressing Lassa Virus-like Particles Protects Mice from Lethal Intra-cerebral Virus Challenge Pathogens (Basel, Switzerland). Aug, 2019 | Pubmed ID: 31466243 Seroprevalence of Human T-lymphotropic Virus HTLV and Its Associated Factors in Donors of a Blood Bank of Medellín-Colombia, 2014-2018 PloS One. Month, 2019 | Pubmed ID: 31404110 HIV Replication in Humanized IL-3/GM-CSF-Transgenic NOG Mice Pathogens (Basel, Switzerland). Mar, 2019 | Pubmed ID: 30871027 T-Cell Response to Viral Hemorrhagic Fevers Vaccines. Jan, 2019 | Pubmed ID: 30678246 Improving the Breadth of the Host's Immune Response to Lassa Virus Pathogens (Basel, Switzerland). Oct, 2018 | Pubmed ID: 30373278 Development of a Serosurveillance Assay for Detection of Necoclí Virus Exposure Archives of Virology. Dec, 2018 | Pubmed ID: 30191374 A Primate Model for Viral Hemorrhagic Fever Methods in Molecular Biology (Clifton, N.J.). Month, 2018 | Pubmed ID: 28986843 Diagnostics for Lassa Fever: Detecting Host Antibody Responses Methods in Molecular Biology (Clifton, N.J.). Month, 2018 | Pubmed ID: 28986826 Modulation of SIV and HIV DNA Vaccine Immunity by Fas-FasL Signaling Viruses. Mar, 2015 | Pubmed ID: 25807052 Transcriptome Analysis of Human Peripheral Blood Mononuclear Cells Exposed to Lassa Virus and to the Attenuated Mopeia/Lassa Reassortant 29 (ML29), a Vaccine Candidate PLoS Neglected Tropical Diseases. Month, 2013 | Pubmed ID: 24069471 Arenavirus Variations Due to Host-specific Adaptation Viruses. Jan, 2013 | Pubmed ID: 23344562 Gene Expression in Primate Liver During Viral Hemorrhagic Fever Virology Journal. Feb, 2009 | Pubmed ID: 19216742 Early Blood Profiles of Virus Infection in a Monkey Model for Lassa Fever Journal of Virology. Aug, 2007 | Pubmed ID: 17522210 Vaccinia Virus Inhibits T Cell Receptor-dependent Responses by Human Gammadelta T Cells The Journal of Infectious Diseases. Jan, 2007 | Pubmed ID: 17152007 The Proline-rich Homeodomain (PRH/HEX) Protein is Down-regulated in Liver During Infection with Lymphocytic Choriomeningitis Virus Journal of Virology. Feb, 2005 | Pubmed ID: 15681447 Monocytes Treated with Human Immunodeficiency Virus Tat Kill Uninfected CD4(+) Cells by a Tumor Necrosis Factor-related Apoptosis-induced Ligand-mediated Mechanism Journal of Virology. Jun, 2003 | Pubmed ID: 12767990 Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models Federico Perdomo-Celis1,2, Sandra Medina-Moreno1, Alonso Heredia1, Harry Davis1, Joseph Bryant1, Juan Carlos Zapata1 1Institute of Human Virology, School of Medicine, University of Maryland, 2Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia JoVE 60315 Immunology and Infection
Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models Federico Perdomo-Celis1,2, Sandra Medina-Moreno1, Alonso Heredia1, Harry Davis1, Joseph Bryant1, Juan Carlos Zapata1 1Institute of Human Virology, School of Medicine, University of Maryland, 2Grupo Inmunovirología, Facultad de Medicina, Universidad de Antioquia JoVE 60315 Immunology and Infection