Junping Yu Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology Chinese Academy of Sciences Biography Publications Institution JoVE Articles Junping Yu has not added a biography. If you are Junping Yu and would like to personalize this page please email our Author Liaison for assistance. Publications A Novel Bacteriophage Endolysin LysAB54 With High Antibacterial Activity Against Multiple Gram-Negative Microbes Frontiers in Cellular and Infection Microbiology. 2021 | Pubmed ID: 33738267 Rapid Genome-wide Sequence Typing of African Swine Fever Virus Based on Alleles Virus Research. Mar, 2021 | Pubmed ID: 33667625 Developing Multiplex DdPCR Assays for SARS-CoV-2 Detection Based on Probe Mix and Amplitude Based Multiplexing Expert Review of Molecular Diagnostics. 01, 2021 | Pubmed ID: 33380245 Development and Evaluation of a Single Dye Duplex Droplet Digital PCR Assay for the Rapid Detection and Quantification of Microorganisms. May, 2020 | Pubmed ID: 32397601 Application of Adaptive Evolution to Improve the Stability of Bacteriophages During Storage Viruses. 04, 2020 | Pubmed ID: 32283683 Ultrasensitive and Selective Detection of Staphylococcus Aureus Using a Novel IgY-based Colorimetric Platform Biosensors & Bioelectronics. Oct, 2019 | Pubmed ID: 31401227 A Novel Double Core-Shell Bionanoparticle Formed by Ferritin and Baculoviral Polyhedrin Journal of Nanoscience and Nanotechnology. Nov, 2019 | Pubmed ID: 31039919 Construction of Lentivirus-Based Reference Material for RT-PCR Detection of Middle East Respiratory Syndrome Coronavirus and Its Application in External Quality Assessment Journal of Nanoscience and Nanotechnology. Sep, 2019 | Pubmed ID: 30961704 Bacteriophages Isolated in China for the Control of Pectobacterium Carotovorum Causing Potato Soft Rot in Kenya Virologica Sinica. Jun, 2019 | Pubmed ID: 30868359 An Insight into DNA Binding Properties of Newly Designed Cationic δ,δ'‑diazacarbazoles: Spectroscopy, AFM Imaging and Living Cells Staining Studies Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy. Mar, 2019 | Pubmed ID: 30557843 Exploring a Phage-based Real-time PCR Assay for Diagnosing Acinetobacter Baumannii Bloodstream Infections with High Sensitivity Analytica Chimica Acta. Dec, 2018 | Pubmed ID: 30442396 Virulence Attenuation and Immune Clearance Mediated by a Phage Lysin-derived Protein The EMBO Journal. 09, 2018 | Pubmed ID: 30037823 Parallel Susceptibility Testing of Bacteria Through Culture-quantitative PCR in 96-well Plates International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases. May, 2018 | Pubmed ID: 29559368 Developing a Bacteriophage Cocktail for Biocontrol of Potato Bacterial Wilt Virologica Sinica. Dec, 2017 | Pubmed ID: 29168148 Accurate Detection of Methicillin-Resistant Staphylococcus Aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR Journal of Clinical Microbiology. 10, 2017 | Pubmed ID: 28724560 Rapid and Selective Detection of E. Coli O157:H7 Combining Phagomagnetic Separation with Enzymatic Colorimetry Food Chemistry. Nov, 2017 | Pubmed ID: 28551244 Combining Phagomagnetic Separation with Immunoassay for Specific, Fast and Sensitive Detection of Staphylococcus Aureus Talanta. Aug, 2017 | Pubmed ID: 28501172 A Novel Chimeric Lysin with Robust Antibacterial Activity Against Planktonic and Biofilm Methicillin-resistant Staphylococcus Aureus Scientific Reports. 01, 2017 | Pubmed ID: 28067286 Rapid Detection of Viral Antibodies Based on Multifunctional Staphylococcus Aureus Nanobioprobes Enzyme and Microbial Technology. Dec, 2016 | Pubmed ID: 27866631 Rapid and Sensitive Detection of HIV-1 P24 Antigen by Immunomagnetic Separation Coupled with Catalytic Fluorescent Immunoassay Analytical and Bioanalytical Chemistry. Sep, 2016 | Pubmed ID: 27351993 Comparison Between a Chimeric Lysin ClyH and Other Enzymes for Extracting DNA to Detect Methicillin Resistant Staphylococcus Aureus by Quantitative PCR World Journal of Microbiology & Biotechnology. Jan, 2016 | Pubmed ID: 26596268 Sensitive and Rapid Detection of Staphylococcus Aureus in Milk Via Cell Binding Domain of Lysin Biosensors & Bioelectronics. Mar, 2016 | Pubmed ID: 26433070 Antibacterial Activity of a Novel Peptide-Modified Lysin Against Acinetobacter Baumannii and Pseudomonas Aeruginosa Frontiers in Microbiology. 2015 | Pubmed ID: 26733995 A Chimeolysin with Extended-spectrum Streptococcal Host Range Found by an Induced Lysis-based Rapid Screening Method Scientific Reports. Nov, 2015 | Pubmed ID: 26607832 Study of the Interactions Between Endolysin and Bacterial Peptidoglycan on S. Aureus by Dynamic Force Spectroscopy Nanoscale. Oct, 2015 | Pubmed ID: 26324763 Simple and Rapid Preparation of Red Fluorescence and Red Color S. Aureus Derived Nanobioparticles for Pathogen Detection Journal of Microbiological Methods. Aug, 2015 | Pubmed ID: 26003440 Aspartate Inhibits Staphylococcus Aureus Biofilm Formation FEMS Microbiology Letters. Apr, 2015 | Pubmed ID: 25687923 Molecular Dissection of Phage Lysin PlySs2: Integrity of the Catalytic and Cell Wall Binding Domains is Essential for Its Broad Lytic Activity Virologica Sinica. Feb, 2015 | Pubmed ID: 25680444 Isolation and Complete Genome Sequence of a Novel Virulent Mycobacteriophage, CASbig Virologica Sinica. Feb, 2015 | Pubmed ID: 25646930 Construction of a Chimeric Lysin Ply187N-V12C with Extended Lytic Activity Against Staphylococci and Streptococci Microbial Biotechnology. Mar, 2015 | Pubmed ID: 25219798 Engineered Bacteriophage Lysins As Novel Anti-infectives Frontiers in Microbiology. 2014 | Pubmed ID: 25360133 Degradation of Methicillin-resistant Staphylococcus Aureus Biofilms Using a Chimeric Lysin Biofouling. 2014 | Pubmed ID: 24742201 Rapid Detection of New Delhi Metallo-β-lactamase Gene and Variants Coding for Carbapenemases with Different Activities by Use of a PCR-based in Vitro Protein Expression Method Journal of Clinical Microbiology. Jun, 2014 | Pubmed ID: 24671780 PCR-based in Vitro Synthesis of Hepatitis C Virus NS3 Protease for Rapid Phenotypic Resistance Testing of Protease Inhibitors Journal of Clinical Microbiology. Apr, 2014 | Pubmed ID: 24452171 Rapid Detection of Mycobacterium Tuberculosis and Pyrazinamide Susceptibility Related to PncA Mutations in Sputum Specimens Through an Integrated Gene-to-protein Function Approach Journal of Clinical Microbiology. Jan, 2014 | Pubmed ID: 24226918 Novel Chimeric Lysin with High-level Antimicrobial Activity Against Methicillin-resistant Staphylococcus Aureus in Vitro and in Vivo Antimicrobial Agents and Chemotherapy. 2014 | Pubmed ID: 24189265 Use of HGluc/tdTomato Pair for Sensitive BRET Sensing of Protease with High Solution Media Tolerance Talanta. May, 2013 | Pubmed ID: 23618151 Effects of Fullerene Derivatives on Bioluminescence and Application for Protease Detection Chemical Communications (Cambridge, England). Nov, 2012 | Pubmed ID: 23037447 Existence of Separate Domains in Lysin PlyG for Recognizing Bacillus Anthracis Spores and Vegetative Cells Antimicrobial Agents and Chemotherapy. Oct, 2012 | Pubmed ID: 22802245 Buffer Enhanced Bioluminescence Resonance Energy Transfer Sensor Based on Gaussia Luciferase for in Vitro Detection of Protease Analytica Chimica Acta. Apr, 2012 | Pubmed ID: 22483217 Study of Inhibition Effect of Herceptin on Interaction Between Heregulin and ErbB Receptors HER3/HER2 by Single-molecule Force Spectroscopy Experimental Cell Research. Oct, 2009 | Pubmed ID: 19497323 Study of Interaction Between Smad7 and DNA by Single-molecule Force Spectroscopy Biochemical and Biophysical Research Communications. Dec, 2008 | Pubmed ID: 18992714 Single-molecule Force Spectroscopy Study of Interaction Between Transforming Growth Factor Beta1 and Its Receptor in Living Cells The Journal of Physical Chemistry. B. Dec, 2007 | Pubmed ID: 17997544 Energy Landscape of Aptamer/protein Complexes Studied by Single-molecule Force Spectroscopy Chemistry, an Asian Journal. Feb, 2007 | Pubmed ID: 17441163 Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification Raphael Nyaruaba1,2,3, Xiaohong Li1, Caroline Mwaliko2,3,4, Changchang Li1, Matilu Mwau5, Nelson Odiwour1,2,3, Elishiba Muturi1,2,3, Caroline Muema1,2,3, Junhua Li1, Junping Yu1, Hongping Wei1 1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute JoVE 62295 Biology
Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification Raphael Nyaruaba1,2,3, Xiaohong Li1, Caroline Mwaliko2,3,4, Changchang Li1, Matilu Mwau5, Nelson Odiwour1,2,3, Elishiba Muturi1,2,3, Caroline Muema1,2,3, Junhua Li1, Junping Yu1, Hongping Wei1 1Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Wuhan Institute of Virology, Chinese Academy of Sciences, 2International College, University of Chinese Academy of Sciences, 3Sino-Africa Joint Research Center, 4CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Chinese Academy of Sciences, 5Center for Infectious and Parasitic Diseases Control Research, Kenya Medical Research Institute JoVE 62295 Biology