Leo Q. Wan Biomedical Engineering & Biological Sciences Rensselaer Polytechnic Institute Biography Publications Institution JoVE Articles Leo Q. Wan Dr. Leo Q. Wan is an associate professor in the Department of Biomedical Engineering at the Rensselaer Polytechnic Institute in Troy, NY. His research focuses on understanding physical biology in tissue development and regeneration and includes Tissue Morphogenesis, Stem Cell Mechanobiology, and Functional Tissue Engineering. He is a pioneer in cell chirality research with bioengineering platforms. His lecture titled “Why are human bodies asymmetrical?”, collaborated with TED-Ed, has over 1 million views. Dr. Wan received his Bachelor’s degree in Mechanics and Mechanical Engineering and his Master's degree in Fluid Mechanics from the University of Science and Technology of China. After completing his Ph.D. in Biomedical Engineering at Columbia University in 2007, he became a postdoctoral scientist in the area of Stem Cells and Tissue Engineering. Leo is a Pew scholar (Class 2013), and a recipient of the NIH Director’s New Innovator Award, National Science Foundation Early Career Award, American Heart Association Scientist Development Grant, and the March of Dimes Basil O’Connor Starter Scholar Research Award. He is a fellow of the American Heart Association (FAHA). Publications Cell Chirality As a Novel Measure for Cytotoxicity Advanced Biology. 01, 2022 | Pubmed ID: 34796704 Effects of Alzheimer's Disease-Related Proteins on the Chirality of Brain Endothelial Cells Cellular and Molecular Bioengineering. Jun, 2021 | Pubmed ID: 34109002 Recent Advances in Cellular and Molecular Bioengineering for Building and Translation of Biological Systems Cellular and Molecular Bioengineering. Aug, 2021 | Pubmed ID: 34055096 Hyperosmolar Ionic Solutions Modulate Inflammatory Phenotype and SGAG Loss in a Cartilage Explant Model Cartilage. 12, 2021 | Pubmed ID: 32975437 Cell Chirality in Cardiovascular Development and Disease APL Bioengineering. Sep, 2020 | Pubmed ID: 32903894 Cardiomyocyte Orientation Modulated by the Numb Family Proteins-N-cadherin Axis is Essential for Ventricular Wall Morphogenesis Proceedings of the National Academy of Sciences of the United States of America. 07, 2019 | Pubmed ID: 31300538 Cell Organelle-based Analysis of Cell Chirality Communicative & Integrative Biology. Month, 2019 | Pubmed ID: 31143366 Identification of Chondrocyte Genes and Signaling Pathways in Response to Acute Joint Inflammation Scientific Reports. 01, 2019 | Pubmed ID: 30643177 Temporal Effects of Cytokine Treatment on Lubricant Synthesis and Matrix Metalloproteinase Activity of Fibroblast-like Synoviocytes Journal of Tissue Engineering and Regenerative Medicine. 01, 2019 | Pubmed ID: 30403832 Lineage-Specific Chiral Biases of Human Embryonic Stem Cells During Differentiation Stem Cells International. Month, 2018 | Pubmed ID: 30627170 In Vitro Microscale Models for Embryogenesis Advanced Biosystems. Jun, 2018 | Pubmed ID: 30533517 Intrinsic Cellular Chirality Regulates Left-right Symmetry Breaking During Cardiac Looping Proceedings of the National Academy of Sciences of the United States of America. 12, 2018 | Pubmed ID: 30459275 Epithelial Cell Chirality Revealed by Three-Dimensional Spontaneous Rotation Proceedings of the National Academy of Sciences of the United States of America. 11, 2018 | Pubmed ID: 30429314 Cell Chirality Regulates Intercellular Junctions and Endothelial Permeability Science Advances. 10, 2018 | Pubmed ID: 30397640 Teratogen Screening with Human Pluripotent Stem Cells Integrative Biology : Quantitative Biosciences from Nano to Macro. 09, 2018 | Pubmed ID: 30095839 Cartilage Metabolism is Modulated by Synovial Fluid Through Metalloproteinase Activity Annals of Biomedical Engineering. Jun, 2018 | Pubmed ID: 29589167 Zonal Variation of MRI-measurable Parameters Classifies Cartilage Degradation Journal of Biomechanics. Dec, 2017 | Pubmed ID: 29126604 CDC42 is Required for Epicardial and Pro-epicardial Development by Mediating FGF Receptor Trafficking to the Plasma Membrane Development (Cambridge, England). 05, 2017 | Pubmed ID: 28465335 Multiaxial Polarity Determines Individual Cellular and Nuclear Chirality Cellular and Molecular Bioengineering. Feb, 2017 | Pubmed ID: 28360944 Cell Chirality: Emergence of Asymmetry from Cell Culture Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences. 12, 2016 | Pubmed ID: 27821525 Sequential Gelation of Tyramine-substituted Hyaluronic Acid Hydrogels Enhances Mechanical Integrity and Cell Viability Medical & Biological Engineering & Computing. Dec, 2016 | Pubmed ID: 27056409 Cellular and Nuclear Alignment Analysis for Determining Epithelial Cell Chirality Annals of Biomedical Engineering. May, 2016 | Pubmed ID: 26294010 Determining Tension-Compression Nonlinear Mechanical Properties of Articular Cartilage from Indentation Testing Annals of Biomedical Engineering. Apr, 2016 | Pubmed ID: 26240062 Effects of Osmolarity on the Spontaneous Calcium Signaling of In Situ Juvenile and Adult Articular Chondrocytes Annals of Biomedical Engineering. Apr, 2016 | Pubmed ID: 26219403 Inhibition of Cell-cell Adhesion Impairs Directional Epithelial Migration on Micropatterned Surfaces Integrative Biology : Quantitative Biosciences from Nano to Macro. May, 2015 | Pubmed ID: 25923643 Zinc Inhibits Hedgehog Autoprocessing: Linking Zinc Deficiency with Hedgehog Activation The Journal of Biological Chemistry. May, 2015 | Pubmed ID: 25787080 Astrocytes Increase ATP Exocytosis Mediated Calcium Signaling in Response to Microgroove Structures Scientific Reports. Month, 2015 | Pubmed ID: 25597401 Large, Stratified, and Mechanically Functional Human Cartilage Grown in Vitro by Mesenchymal Condensation Proceedings of the National Academy of Sciences of the United States of America. May, 2014 | Pubmed ID: 24778247 High-throughput Cell Aggregate Culture for Stem Cell Chondrogenesis Methods in Molecular Biology (Clifton, N.J.). Month, 2014 | Pubmed ID: 24676785 Carbon Nanotube-induced Loss of Multicellular Chirality on Micropatterned Substrate is Mediated by Oxidative Stress ACS Nano. Mar, 2014 | Pubmed ID: 24559311 Micropatterning of Cells Reveals Chiral Morphogenesis Stem Cell Research & Therapy. Apr, 2013 | Pubmed ID: 23672821 A Micropatterning Assay for Measuring Cell Chirality Haokang Zhang1,2, Kacey Ronaldson-Bouchard3, Gordana Vunjak-Novakovic3,4, Leo Q. Wan1,2,5,6 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, 3Department of Biomedical Engineering, Columbia University, 4Department of Medicine, Columbia University, 5Department of Biological Sciences, Rensselaer Polytechnic Institute, 6Center for Modeling, Simulation, and Imaging in Medicine, Rensselaer Polytechnic Institute JoVE 63105 Bioengineering
A Micropatterning Assay for Measuring Cell Chirality Haokang Zhang1,2, Kacey Ronaldson-Bouchard3, Gordana Vunjak-Novakovic3,4, Leo Q. Wan1,2,5,6 1Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 2Center for Biotechnology & Interdisciplinary Studies, Rensselaer Polytechnic Institute, 3Department of Biomedical Engineering, Columbia University, 4Department of Medicine, Columbia University, 5Department of Biological Sciences, Rensselaer Polytechnic Institute, 6Center for Modeling, Simulation, and Imaging in Medicine, Rensselaer Polytechnic Institute JoVE 63105 Bioengineering